IgA ELISA 试剂盒
Quick Overview for IgA ELISA 试剂盒 (ABIN5067962)
抗原
See all IgA ELISA试剂盒适用
检测方法
应用范围
样品类型
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原理
- The Human IgA ELISA Kit is an in vitro immunoassay for research use in the quantification of human IgA circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of human cells.
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Analytical Method
- Quantitative
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灵敏度
- 20 ng/mL
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产品特性
- IgA, Human, ELISA Kit
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组件
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- Coated Microwell Plate: 12x8-well strips
- Human IgA Positive Control (ng/ml), 1x 650ul
- Human IgA Standard 50ng/ml, 1x 650ul
- Human IgA Standard 100ng/ml, 1x 650ul
- Human IgA Standard 250ng/ml, 1x 650ul
- Human IgA Standard 500ng/ml, 1x 650ul
- Human IgA Standard 1000ng/ml, 1x 650ul
- IgA (HRP) Conjugate, 100X, 1x 150ul
- Sample Diluent, 20X, 1x10ml
- Wash Solution, 100X, 1x10ml
- TMB Substrate: 1x12ml
- Stop Solution: 1x12ml
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IgA ELISA Kit
适用: Cow Colorimetric Competition ELISA 0.39 μg/mL - 25 μg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
IgA ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Plasma, Serum
IgA ELISA Kit适用: 人 Colorimetric Sandwich ELISA 0.14 ng/mL - 100 ng/mL Plasma, Saliva, Serum
IgA ELISA Kit适用: 绵羊 Colorimetric Competition ELISA 3.75 μg/mL - 60 μg/mL Plasma, Serum
IgA ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
IgA ELISA Kit适用: 小鸡 Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Plasma, Serum
IgA ELISA Kit适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
IgA ELISA Kit适用: 小鸡 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
IgA ELISA Kit适用: 犬 Colorimetric Sandwich ELISA 31.25 ng/mL - 1000 ng/mL Plasma, Serum, Urine
IgA ELISA Kit适用: 小鸡 Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
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实验流程
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Principle:
- The Human IgA ELISA kit is based on the binding of human IgA in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of IgA present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of IgA in samples and control is calculated from a curve of standards containing known concentrations of IgA.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C
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- IgA
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物质类
- Antibody
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