Defensin beta 3 ELISA 试剂盒
This 人 Defensin beta 3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 Defensin beta 3.
Quick Overview for Defensin beta 3 ELISA 试剂盒 (ABIN5067941)
抗原
See all Defensin beta 3 (DEFB3) ELISA试剂盒
Defensin beta 3 (DEFB3)
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
12.5-400 pg/mL
应用范围
ELISA
样品类型
Serum, Cell Culture Samples, Saliva, Tissue Samples
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最低检测浓度
- 12.5 pg/mL
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原理
- The Human Beta Defensin 3 (BD-3) ELISA Kit is an in vitro immunoassay for research use in the quantification of BD-3 in cultures of human cells and in appropriately qualified samples from serum, saliva, or other tissue fluids. The kit is suitable for testing a variety of sample types, in accordance with appropriate validation of linearity and recovery.
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Analytical Method
- Quantitative
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灵敏度
- 20 pg/mL
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产品特性
- Beta Defensin 3, Human, ELISA Kit (BD3)
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组件
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- Microwell Strips, 1x96 wells (12x8-well strips). Coated with purified anti-human BD3 antibodies. Return unused strips to the pouch with desiccant, re-seal and store refrigerated.
- Standard, 400pg/ml, 2x1 vial. Recombinant human BD3. Supplied as a lyophilized powder from buffer, protein stabilizers.
- Ab (Biotin), 100X, 1x150ul. Biotinylated anti-human BD3. Supplied as a liquid in buffer, protein, detergents, 0.05% Bronidox.
- Streptavidin (HRP), 100X, 1x150ul. Supplied as a liquid in buffer with protein, detergents, 0.05% Bronidox.
- Sample Diluent, 10X, 1x10ml.
- Biotin, HRP Diluent, 1x22ml. Supplied as a liquid in buffer, protein, anti-microbials.
- Wash Solution, 100X, 1x10ml.
- TMB Solution, 1x12ml. Chromogenic substrate for HRP containing TMB and peroxide.
- Stop Solution (1% O4), 1x12ml. 1% sulfuric acid. Caution: Corrosive!
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Defensin beta 3 (DEFB3) ELISA Kit
DEFB3 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Defensin beta 3 (DEFB3) ELISA KitDEFB3 适用: 人 Colorimetric Sandwich ELISA
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板类型
- Pre-coated
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实验流程
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Principle:
- The assay is based on the binding of human Beta Defensin 3 in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to biotin, which then binds to a streptavidin (HRP). After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of BD-3 present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of BD-3 in samples is calculated from a standard curve of purified recombinant human BD-3 of designated concentration.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C
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- Defensin beta 3 (DEFB3)
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别名
- Beta Defensin 3
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途径
- Production of Molecular Mediator of Immune Response
抗原 See all Defensin beta 3 (DEFB3) ELISA试剂盒
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