beta Amyloid ELISA 试剂盒
This 人 beta Amyloid ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 beta Amyloid in Cerebrospinal Fluid, Tissue Homogenate 和 Tissue Culture Medium.
Quick Overview for beta Amyloid ELISA 试剂盒 (ABIN5067940)
抗原
See all beta Amyloid (Abeta) ELISA试剂盒
beta Amyloid (Abeta)
(Amyloid beta (Abeta))
抗原表位
AA 1-40
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Cerebrospinal Fluid, Tissue Homogenate, Tissue Culture Medium
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原理
- The Human beta Amyloid 1-40 (Hu A beta40) ELISA kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for the NH2-terminus of Hu b-amyloid has been coated onto the wells of the microtiter strips provided. During the first incubation, standards of known Hu b-amyloid 40 content, controls, and unknown samples are pipetted into the wells and co-incubated with a rabbit antibody specific for the COOH-terminus of the 1-40 b-amyloid sequence. This COOH- terminal sequence is created upon cleavage of the analyzed precursor. Bound rabbit antibody is detected by the use of a horseradish peroxidase-labeled anti-rabbit antibody. After washing, horseradish peroxidase-labeled anti-rabbit antibody (enzyme) is added. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Hu A beta40 present in the original specimen.
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Analytical Method
- Quantitative
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交叉反应 （详细）
- Calculated cross reactivity: Hu
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灵敏度
- > 6 pg/mL
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产品特性
- β-Amyloid, aa1-40, Human, ELISA Kit
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组件
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- * beta Amyloid Standard, 1x1vial
- Standard Diluent Buffer, 1x60ml
- Microtiter Plate, 1x96 wells
- beta Amyloid , Pab 1x6ml
- IgG (HRP) (100X), 1x125ul
- HRP Diluent, 1x25ml
- Wash Buffer (25X), 1x100ml
- TMB, 1x25ml
- Stop Solution, 1x25ml
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Amyloid beta (Abeta) ELISA Kit
Abeta 适用: 人 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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限制
- 仅限研究用
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储存条件
- 4 °C,-80 °C
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储存方法
- 4°C/-70°C
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- beta Amyloid (Abeta) (Amyloid beta (Abeta))
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别名
- beta-Amyloid
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途径
- Inflammasome
抗原 See all beta Amyloid (Abeta) ELISA试剂盒
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