Pepsin ELISA 试剂盒
Quick Overview for Pepsin ELISA 试剂盒 (ABIN5067908)
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See all Pepsin ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- PP (Pepsin) ELISA Kit is for the quantitative detection of Pepsin in porcine serum, plasma, tissue homogenates and other biological fluids.
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Analytical Method
- Quantitative
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灵敏度
- 0.938 ng/mL
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产品特性
- PP (Pepsin) ELISA Kit (Porcine)
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组件
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- *358254A: Microtiter Strips, 1x96 wells (8x12 wells).
- *358254B: Standard, 2x1 vial
- 358254C: Sample/Standard Dilution Buffer, 1x20ml
- 358254D: Antibody (Biotin) (Concentrated), 1x120ul
- 358254E: Antibody Dilution Buffer, 1x10ml
- 358254F: Streptavidin (HRP) (SABC), 1x120ul
- 358254G: SABC Dilution Buffer, 1x10ml
- 358254H: TMB Substrate, 1x10ml
- 358254J: Stop Solution, 1x10ml
- 358254K: Wash Buffer, 25X, 1x30ml
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Pepsin ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Pepsin ELISA Kit适用: 人 Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pepsin ELISA Kit适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Pepsin ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Pepsin ELISA Kit适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Pepsin ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pepsin ELISA Kit适用: 小鼠 Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pepsin ELISA KitInstant 适用: 人 Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pepsin ELISA KitSmall Sample 适用: 小鼠 Colorimetric Sandwich ELISA 1.23 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pepsin ELISA Kit适用: Pig Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum
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板类型
- Pre-coated
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实验流程
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Principle:
- This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate pre-coated with an antibody specific to Pepsin. Standards and samples are added to the appropriate wells, then incubated. Aspirate. Do not wash the plate! The Antibody (Biotin) is added to all the wells. The plate is incubated and then washed. Streptavidin (HRP) is added to each microplate well and incubated then washed. Then the TMB substrate solution is added and incubated. After the TMB substrate solution is added, only those wells that contain Pepsin, the antibody (Biotin) and Streptavidin (HRP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pepsin in the sample is then determined by comparing the O.D. of the sample to the standard curve.
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实验精密度
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Precision:
- Intra-Assay CV: <8%
- Inter-Assay CV: <10%
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C/-20°C
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- Pepsin
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别名
- PP (Pepsin)
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