Phenylalanine Hydroxylase ELISA 试剂盒
Quick Overview for Phenylalanine Hydroxylase ELISA 试剂盒 (ABIN5067907)
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检测方法
实验类型
应用范围
样品类型
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原理
- PAH (Phenylalanine Hydroxylase) ELISA Kit is a quantitative sandwich assay for the detection of PAH in human serum, plasma, tissue homogenates and other biological fluids
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Analytical Method
- Quantitative
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灵敏度
- 46.875 pg/mL
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产品特性
- PAH (Phenylalanine Hydroxylase) ELISA Kit (Human)
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组件
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- *357883A: Microtiter Strips, 1x96 wells (8x12 wells).
- *357883B: Standard, 2x1 vial
- 357883C: Sample/Standard Dilution Buffer, 1x20ml
- 357883D: Antibody (Biotin) (Concentrated), 1x120ul
- 357883E: Antibody Dilution Buffer, 1x10ml
- 357883F: Streptavidin (HRP) (SABC), 1x120ul
- 357883G: SABC Dilution Buffer, 1x10ml
- 357883H: TMB Substrate, 1x10ml
- 357883J: Stop Solution, 1x10ml
- 357883K: Wash Buffer, 25X, 1x30ml
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Phenylalanine Hydroxylase ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Phenylalanine Hydroxylase ELISA Kit适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Phenylalanine Hydroxylase ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 23.5 pg/mL - 1500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Phenylalanine Hydroxylase ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
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Principle:
- This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate pre-coated with an antibody specific to PAH. Standards and samples are added to the appropriate wells, then incubated. Aspirate. Do not wash the plate! The Antibody (Biotin) is added to all the wells. The plate is incubated and then washed. Streptavidin (HRP) is added to each microplate well and incubated then washed. Then the TMB substrate solution is added and incubated. After the TMB substrate solution is added, only those wells that contain PAH, the antibody (Biotin) and Streptavidin (HRP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PAH in the sample is then determined by comparing the O.D. of the sample to the standard curve.
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实验精密度
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Precision:
- Intra-Assay CV: <8%
- Inter-Assay CV: <10%
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C/-20°C
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- Phenylalanine Hydroxylase
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别名
- PAH (Phenylalanine Hydroxylase)
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物质类
- Chemical
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