Transferrin Receptor ELISA 试剂盒
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- 抗原 See all Transferrin Receptor (TFRC) ELISA试剂盒
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human sTfR ELISA is a sandwich enzyme immunoassay for the quantitative measurement of soluble human transferrin receptor in serum, plasma and tissue culture medium.
- 样品类型
- Cell Culture Lysate, Plasma, Serum
- Analytical Method
- Quantitative
- 灵敏度
- 2 ng/mL
- 产品特性
- Transferrin Receptor, soluble, Human, ELISA Kit (TfR1, TFRC, CD71, TR, Tfr, TfnrR, sTfR)
- 组件
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- Microtiter Strips, 1x 96 wells, coated with anti-Hu sTfR Mab
- Mab (HRP), 1x13ml
- Standard, 2ug/ml, 1x100ul
- Standard, 1ug/ml, 1x100ul
- Standard, 0.5ug/ml, 1x.100ul
- Standard, 0.2ug/ml, 1x100ul
- Standard, 0.1ug/ml, 1x100ul
- Standard, 0.05ug/ml, 1x100ul
- Quality Control, High, 50X, (5..3ug /ml), 1x50ul
- Quality Control, Low, 50X, (1..0ug/ml), 1x50ul
- Dilution Buffer, 2x13ml
- Wash Solution, 10X, 1x100ml
- Substrate Solution (TMB), 1x13ml
- Stop Solution (0.2M O4), 1x13ml
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- 板类型
- Pre-coated
- 实验流程
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Principle:
- Standards, quality controls and samples of sera are incubated in microtitration wells coated with monoclonal anti-human sTfR antibody. After a thorough wash, monoclonal anti-human sTfR antibody labeled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody'sTfR complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate H2O2 and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow color product is measured spectrophotometrically at 450nm. The absorbance is proportional to the concentration of sTfR. A standard curve is constructed by plotting absorbance values versus recombinant sTfR concentrations of standards, and concentrations of unknown samples are determined using this standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- 4°C
-
- 抗原 See all Transferrin Receptor (TFRC) ELISA试剂盒
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
- 别名
- Transferrin Receptor (TFRC 产品)
- 别名
- Trfr ELISA Kit, LOC100219280 ELISA Kit, tfr ELISA Kit, cd71 ELISA Kit, tfr1 ELISA Kit, trfr ELISA Kit, CD71 ELISA Kit, T9 ELISA Kit, TFR ELISA Kit, TFR1 ELISA Kit, TR ELISA Kit, TRFR ELISA Kit, p90 ELISA Kit, 2610028K12Rik ELISA Kit, AI195355 ELISA Kit, AI426448 ELISA Kit, AU015758 ELISA Kit, E430033M20Rik ELISA Kit, Mtvr-1 ELISA Kit, Mtvr1 ELISA Kit, transferrin receptor ELISA Kit, TFRC ELISA Kit, Tfrc ELISA Kit, tfrc ELISA Kit
- 途径
- Transition Metal Ion Homeostasis
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