Transferrin Receptor ELISA 试剂盒
Quick Overview for Transferrin Receptor ELISA 试剂盒 (ABIN5067899)
抗原
See all Transferrin Receptor (TFRC) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- Human sTfR ELISA is a sandwich enzyme immunoassay for the quantitative measurement of soluble human transferrin receptor in serum, plasma and tissue culture medium.
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Analytical Method
- Quantitative
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灵敏度
- 2 ng/mL
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产品特性
- Transferrin Receptor, soluble, Human, ELISA Kit (TfR1, TFRC, CD71, TR, Tfr, TfnrR, sTfR)
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组件
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- Microtiter Strips, 1x 96 wells, coated with anti-Hu sTfR Mab
- Mab (HRP), 1x13ml
- Standard, 2ug/ml, 1x100ul
- Standard, 1ug/ml, 1x100ul
- Standard, 0.5ug/ml, 1x.100ul
- Standard, 0.2ug/ml, 1x100ul
- Standard, 0.1ug/ml, 1x100ul
- Standard, 0.05ug/ml, 1x100ul
- Quality Control, High, 50X, (5..3ug /ml), 1x50ul
- Quality Control, Low, 50X, (1..0ug/ml), 1x50ul
- Dilution Buffer, 2x13ml
- Wash Solution, 10X, 1x100ml
- Substrate Solution (TMB), 1x13ml
- Stop Solution (0.2M O4), 1x13ml
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Transferrin Receptor (p90, CD71) (TFRC) ELISA Kit
TFRC 适用: 小鼠 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Transferrin Receptor (p90, CD71) (TFRC) ELISA KitTFRC 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Transferrin Receptor (p90, CD71) (TFRC) ELISA KitTFRC 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Transferrin Receptor (p90, CD71) (TFRC) ELISA KitTFRC 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Transferrin Receptor (p90, CD71) (TFRC) ELISA KitTFRC 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Transferrin Receptor (p90, CD71) (TFRC) ELISA KitTFRC 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Transferrin Receptor (p90, CD71) (TFRC) ELISA KitTFRC 适用: 小鼠 Colorimetric Competition ELISA 2.5-50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
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Principle:
- Standards, quality controls and samples of sera are incubated in microtitration wells coated with monoclonal anti-human sTfR antibody. After a thorough wash, monoclonal anti-human sTfR antibody labeled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody'sTfR complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate H2O2 and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow color product is measured spectrophotometrically at 450nm. The absorbance is proportional to the concentration of sTfR. A standard curve is constructed by plotting absorbance values versus recombinant sTfR concentrations of standards, and concentrations of unknown samples are determined using this standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C
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- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
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别名
- Transferrin Receptor
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途径
- Transition Metal Ion Homeostasis
抗原 See all Transferrin Receptor (TFRC) ELISA试剂盒
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