Resistin ELISA 试剂盒
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- 抗原 See all Resistin (RETN) ELISA试剂盒
- Resistin (RETN)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Resistin ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human Resistin in serum, plasma and tissue culture medium.
- 样品类型
- Plasma, Serum, Tissue Culture Medium
- Analytical Method
- Quantitative
- 灵敏度
- 0.012 ng/mL
- 产品特性
- Resistin, Human, ELISA Kit
- 组件
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- Microtiter Plate, 1x96wells
- Standard, 1x1vial
- High Control, 1x1vial
- Low Control, 1x1vial
- Pab (Biotin), 1x13ml
- Streptavidin (HRP), 1x13ml
- Dilution Buffer, 1x10ml
- Wash Solution (10X), 1x100ml
- Substrate Solution (TMB), 1x13ml
- Stop Solution (O4), 1x13ml
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- 实验流程
- Principle: Standards or samples are incubated with a rabbit polyclonal anti-human resistin antibody coated in microtiter wells. After one-hour incubation and a washing, biotin-labeled polyclonal anti-human resistin antibody is added and incubated with captured resistin. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of resistin. A standard curve is constructed by plotting absorbance values versus resistin concentrations of standards. Concentrations of unknown samples are determined using this standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- 4°C
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- 抗原 See all Resistin (RETN) ELISA试剂盒
- Resistin (RETN)
- 别名
- Resistin (RETN 产品)
- 别名
- RETN ELISA Kit, ADSF ELISA Kit, FIZZ3 ELISA Kit, RETN1 ELISA Kit, RSTN ELISA Kit, XCP1 ELISA Kit, Fizz3 ELISA Kit, Rstn ELISA Kit, Xcp4 ELISA Kit, resistin ELISA Kit, RETN ELISA Kit, Retn ELISA Kit
- 途径
- Feeding Behaviour, Smooth Muscle Cell Migration
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