Melatonin Sulfate ELISA 试剂盒
This 人 Melatonin Sulfate ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 Melatonin Sulfate.
Quick Overview for Melatonin Sulfate ELISA 试剂盒 (ABIN5067888)
抗原
See all Melatonin Sulfate (MS) products
Melatonin Sulfate (MS)
适用
人
检测方法
Colorimetric
实验类型
Competition ELISA
应用范围
ELISA
样品类型
Urine
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原理
- Enzyme immunoassay for the quantitative determination of melatonin sulfate in human urine.
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Analytical Method
- Quantitative
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灵敏度
- 1 ng/mL
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产品特性
- Melatonin Sulfate, Human (6-Hydroxymelatonin Sulfate, 6-Sulfatoxymelatonin) ELISA Kit
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组件
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- Microtiter Plate, 1x96 wells
- PAB, 1x200ul
- Melatonin Sulfate (HRP), 40x, 1x200ul
- Standard, 0ng/ml, 1x100ul
- Standard, 1.7ng/ml, 1x100ul
- Standard, 5.2ng/ml, 1x100ul
- Standard, 15.6ng/ml, 1x100ul
- Standard, 46.7ng/ml, 1x100ul
- Standard, 140ng/ml, 1x100ul
- 0: Standard, 420ng/ml, 1x100ul
- 1: Low Control 1, 1x100ul
- 2: High Control 2, 1x100ul
- 3: Assay Buffer, 1x60ml
- 4: Wash Buffer, 20x, 1x50ml
- 5: TMB Substrate, 1x12ml
- 6: Stop Solution, 1M O4, 1x12ml
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Melatonin Sulfate (MS) ELISA Kit
MS 适用: 人 Colorimetric Competition ELISA 4.69 ng/mL - 300 ng/mL Cell Culture Supernatant, Plasma, Serum
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实验流程
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Principle:
- Solid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the wells are washed to stop the competition reaction. After the substrate reaction the intensity of the developed color is inversely proportional to the amount of the antigen in the sample. Results of samples can be determined directly using the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C
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- Melatonin Sulfate (MS)
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别名
- Melatonin Sulfate
抗原 See all Melatonin Sulfate (MS) products
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