Endothelin 1 ELISA 试剂盒
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Quick Overview for Endothelin 1 ELISA 试剂盒 (ABIN5067887)
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See all Endothelin 1 (EDN1) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- Endothelin-1, Human ELISA Kit is a 4.5 hour solid phase ELISA designed to measure ET-1 in cell culture supernates, serum, plasma, and urine. It contains synthetic ET-1 and antibodies raised against synthetic ET-1. This immunoassay has been shown to accurately quantitate synthetic and naturally occurring ET-1.
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Analytical Method
- Quantitative
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灵敏度
- 0.087 pg/mL
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产品特性
- Endothelin-1, ELISA Kit (Human)
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组件
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- Microtiter Strips, 1x96 wells
- Endothelin-1 Mab (HRP), 1x21ml
- Standard, 1x1vial
- Assay Diluent, 1x18ml
- Calibrator Diluent, 1x21ml
- Wash Buffer, 25X, 1x21ml
- Color Reagent A, 1x12ml
- Color Reagent B, 1x12ml
- Stop Solution, 2N O4, 1x6ml
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Endothelin 1 (EDN1) ELISA Kit
EDN1 适用: 人 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Saliva, Serum, Urine
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 兔 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 大鼠 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: Pig Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 小鼠 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 人 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 豚鼠 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 pg/mL - 50 pg/mL Cell Culture Supernatant, Plasma, Serum
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 人 Colorimetric Sandwich ELISA 1.25 pg/mL - 80 pg/mL Cell Culture Supernatant, Plasma, Serum
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 大鼠 Colorimetric Sandwich ELISA 1.25 pg/mL - 80 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
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Principle:
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for ET-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ET-1 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for ET-1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ET-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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实验精密度
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Precision:
- Intra-assay Precision:
- Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. ,Precision:
- Three samples of known concentration were tested in 40 separate assays to assess inter-assay precision.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C
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- Endothelin 1 (EDN1)
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别名
- Endothelin-1
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途径
- Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size
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