Testosterone CLIA Kit
Quick Overview for Testosterone CLIA Kit (ABIN504756)
抗原
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检测方法
实验类型
应用范围
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原理
- Competitive Enzyme Immunoassay (TYPE 7): The essential reagents required for a enzyme immunoassay include antibody, enzyme-antigen conjugate and native antigen. Upon mixing biotinylated antibody, enzyme-antigen conjugate and a serum containing the native free antigen, a competition reaction results between the native free antigen and the enzyme-antigen conjugate for a limited number of antibody binding sites.
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Analytical Method
- Quantitative
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产品特性
- The Quantitative Determination of Free Testosterone Concentration in Human Serum or Plasma by a Microplate Chemiluminescence Immunoassay (CLIA)
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Testosterone ELISA Kit
适用: Various Species Colorimetric Competition ELISA 49.4 pg/mL - 4000 pg/mL Plasma, Serum
Testosterone ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 0.13-25.6 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Testosterone ELISA Kit适用: Various Species Colorimetric Competition ELISA 0.31 ng/mL - 20 ng/mL Plasma, Saliva, Serum, Urine
Testosterone ELISA KitHigh Sensitivity 适用: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Testosterone ELISA Kit适用: Various Species Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum
Testosterone ELISA KitSmall Sample 适用: Various Species Colorimetric Competition ELISA 370.4 pg/mL - 30000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Testosterone ELISA Kit适用: 人 Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Testosterone ELISA Kit适用: Various Species Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Testosterone ELISA Kit适用: Pig Colorimetric Competition ELISA 0.1-20 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- All products that contain human serum have been found to be non-reactive for Hepatitis B Surface Antigen, HIV 1&2 and HCV Antibodies by FDA required tests. Since no known test can offer complete assurance that infectious agents are absent, all human serum products should be handled as potentially hazardous and capable of transmitting disease. Good laboratory procedures for handling blood products can be found in the Center for Disease Control / National Institute of Health, "Biosafety in Microbiological and Biomedical Laboratories," 2nd Edition, 1988, HHS Publication No. (CDC) 88-8395.
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板类型
- Pre-coated
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实验流程
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Specimien Collection and Preparation:
The specimens shall be blood, serum or plasma in type and the usual precautions in the collection of venipuncture samples should be observed. For accurate comparison to established normal values, a fasting morning serum sample should be obtained. The blood should be collected in a plain redtop venipuncture tube or (for plasma) in evacuated tube(s) containing heparin. Allow the blood to clot for serum samples. Centrifuge the specimen to separate the serum or plasma from the cells. Samples may be refrigerated at 2_x001E_8oC for a maximum period of five (5) days. If the specimen(s) cannot be assayed within this time, the sample(s) may be stored at temperatures of _x001E_20oC for up to 30 days. Avoid repetitive freezing and thawing. When assayed in duplicate, 0.040ml of the specimen is required.Reagent Preparation:
1. Wash Buffer Dilute contents of wash solution to 1000ml with distilled or deionized water in a suitable storage container. Diluted buffer can be stored at room temperature (20-27(C) for up to 60 days. 2. Working Signal Reagent Solution - Store at 2 - 8(C. Determine the amount of reagent needed and prepare by mixing equal portions of Signal Reagent A and Signal Reagent B in a clean container. For example, add 1 ml of A and 1ml of B per two (2) eight well strips (A slight excess of solution is made). Discard the unused portion if not used within 36 hours after mixing. If complete utilization of the reagents is anticipated, within the above time constraint, pour the contents of Signal Reagent B into Signal Reagent A and label accordingly. _x000E_Test Procedure:
Before proceeding with the assay, bring all reagents, serum references and controls to room temperature (20 - 27(C). 1. Format the microplates wells for each serum reference, control and patient specimen to be assayed in duplicate. Replace any unused microwell strips back into the aluminum bag, seal and store at 2-8(C. 2. Pipette 0.020 ml (20L) of the appropriate serum reference, control or specimen into the assigned well. 3. Add 0.050 ml (50l) of the Free Testosterone Tracer Reagent to all wells. 4. Swirl the microplate gently for 20-30 seconds to mix. 5. Add 0.050 ml (50l) of Free Testosterone Biotin Reagent to all wells. 6. Swirl the microplate gently for 20-30 seconds to mix. 7. Cover and incubate for 45 minutes at room temperature. 8. Discard the contents of the microplate by decantation or aspiration. If decanting, blot the plate dry with absorbent paper. 9. Add 350l of wash buffer (see Reagent Preparation Section), decant (tap and blot) or aspirate. Repeat four (4) additional times for a total of five (5) washes. An automatic or manual plate washer can be used. Follow the manufacturers instruction for proper usage. If a squeeze bottle is employed, fill each well by depressing the container (avoiding air bubbles) to dispense the wash. Decant the wash and repeat four (4) additional times. 10. Add 0.100 ml (100l) of working signal reagent solution to all wells (see Reagent Preparation Section). Always add reagents in the same order to minimize reaction time differences between wells. 11. Incubate at room temperature for five (5) minutes in the dark. 12. Read the relative light units in each well with a Chemiluminescence microplate reader for 0.5-1.0 seconds. The results should be read within 30 minutes after adding the working Signal Reagent. -
限制
- 仅限研究用
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- Testosterone
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别名
- Testosterone (Free)
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背景
- Testosterone, (17(-Hydroxy-4-androstene-3-one), a C19 steroid, is the most potent naturally secreted androgen1. In normal post pubertal males, testosterone is secreted primarily by the testes with only a small amount derived from peripheral conversion of 4-Androstene-3, 17-dione (ASD)2. In adult women, it has been estimated that over 50% of serum testosterone is derived from peripheral conversion of ASD secreted by the adrenal and ovary, with the remainder from direct secretion of testosterone by these glands. In the male, testosterone is mainly synthesized in the interstitial Leydig cells and the testis, and is regulated by the interstitial cell stimulating hormone (ICSH), or luteinizing hormone (LH) of the anterior pituitary (the female equivalent of ICSH)3. Testosterone is responsible for the development of secondary sex characteristics, such as the accessory sex organs, the prostate, seminal vesicles and the growth of facial, pubic and auxiliary hair. Testosterone measurements have been very helpful in evaluating hypogonadal states. Increased testosterone levels in males can be found in complete androgen resistance (testicular feminization). Common causes of decreased testosterone levels in males include: hypogonadism, orchidectomy, estrogen therapy, Klinefelter's syndrome, hypopituitarism, and hepatic cirrhosis2-4. In the female, testosterone levels are normally found to be much lower than those encountered in the healthy male. Testosterone in the female comes from three sources. It is secreted in small quantities by both the adrenal glands and the ovaries, and in healthy women 5060% of the daily testosterone production arises from peripheral metabolism of prohormone, chiefly androstenedione. Common causes of increased serum testosterone levels in females include polycystic ovaries (Stein-Leventhal syndrome), ovarian tumors, adrenal tumors and adrenal hyperplasia. Virilization in women is associated with the administration of androgens and endogenous overproduction of testosterone. There appears to be a correlation between serum testosterone levels and the degree of virilization in women, although approximately 25% of women with varying degrees of virilism have serum testosterone levels that fall within the female reference range. The majority of testosterone is bound to transport proteins, weakly bound to albumin and cortisol binding protein (25-65% females - 45-85% males) and tightly bound to sex hormone-binding globulin (SHBG) (females 35-75% - males 14-50%)8. A small fraction exist as unbound or free testosterone, however this form is biologically active. Therefore, the free hormone concentration is a better indicator of biological activity than total testosterone.
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