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IL-13 ELISA 试剂盒

IL13 适用: 人 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Plasma, Serum
产品编号 ABIN5026963
发货至: 中国
  • 抗原 See all IL-13 (IL13) ELISA试剂盒
    IL-13 (IL13) (Interleukin 13 (IL13))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    15.6-1000 pg/mL
    最低检测浓度
    15.6 pg/mL
    应用范围
    ELISA
    原理
    This immunoassay kit allows for the in vitro quantitative determination of Human IL-13 concentrations in serum, plasma and other biological fluids.
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    灵敏度
    < 9.4 pg/mL
    组件
    plate, standard, diluent, antibodies
    试剂未包括
    pipettes, tubes, reader, incubator
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    Discover our top product IL13 ELISA Kit
  • 样本量
    100 μL
    实验时间
    3 - 4 h
    板类型
    Pre-coated
    实验流程
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IL-13 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-13 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-13 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human IL-13 can be calculated using the standard curve.
    限制
    仅限研究用
  • 注意事项
    protect your eyes
    储存条件
    4 °C
  • 抗原 See all IL-13 (IL13) ELISA试剂盒
    IL-13 (IL13) (Interleukin 13 (IL13))
    别名
    IL-13 (Interleukin 13) (IL13 产品)
    别名
    IL13 ELISA Kit, IL-13 ELISA Kit, P600 ELISA Kit, Il-13 ELISA Kit, L13 ELISA Kit, interleukin 13 ELISA Kit, IL13 ELISA Kit, Il13 ELISA Kit
    途径
    JAK/STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
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