IL32 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for IL32 ELISA 试剂盒 (ABIN5026960)
抗原
See all IL32 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 15.6 pg/mL
-
原理
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
-
Analytical Method
- Quantitative
-
灵敏度
- 9.4 pg/mL
-
产品特性
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-32 concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL
-
组件
- plate, standard, diluent, antibodies
-
试剂未包括
- pipettes, tubes, reader, incubator
-
-
Interleukin 32 (IL32) ELISA Kit
IL32 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 32 (IL32) ELISA KitIL32 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 32 (IL32) ELISA KitIL32 适用: 人 Colorimetric Sandwich ELISA 78.1 pg/mL – 5000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 32 (IL32) ELISA KitIL32 适用: 人 AA 31-234 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 32 (IL32) ELISA KitIL32 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 32 (IL32) ELISA KitIL32 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
-
样本量
- 100 μL
-
实验时间
- 3 - 4 h
-
板类型
- Pre-coated
-
实验流程
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IL-32 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-32 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-32 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human IL-32 can be calculated using the standard curve.
-
限制
- 仅限研究用
-
-
-
注意事项
- protect your eyes
-
储存条件
- 4 °C
-
储存方法
- Please refer to the Manual
-
-
- IL32 (Interleukin 32 (IL32))
-
别名
- IL-32 (Interleukin 32)
抗原 See all IL32 ELISA试剂盒
-
