alpha Fetoprotein ELISA 试剂盒
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Quick Overview for alpha Fetoprotein ELISA 试剂盒 (ABIN5026955)
抗原
See all alpha Fetoprotein (AFP) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.313 ng/mL
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原理
- This assay employs an antibody specific for Anti-human AFP coated on a 96-well plate.
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Analytical Method
- Quantitative
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灵敏度
- 0.188 ng/mL
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产品特性
- This immunoassay kit allows for the in vitro quantitative determination of Human AFP concentrations in serum, plasma. Detection Range: 0.313 - 20 ng/mL Sensitivity: < 0.188 ng/mL
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组件
- plate, standard, diluent, antibodies
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试剂未包括
- pipettes, tubes, reader, incubator
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alpha-Fetoprotein (AFP) ELISA Kit
AFP 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
alpha-Fetoprotein (AFP) ELISA KitAFP 适用: 人 Colorimetric Sandwich ELISA 1.563-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
alpha-Fetoprotein (AFP) ELISA KitAFP 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
alpha-Fetoprotein (AFP) ELISA KitAFP 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
alpha-Fetoprotein (AFP) ELISA KitAFP 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum
alpha-Fetoprotein (AFP) ELISA KitAFP 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
alpha-Fetoprotein (AFP) ELISA KitAFP 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
alpha-Fetoprotein (AFP) ELISA KitAFP 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
alpha-Fetoprotein (AFP) ELISA KitAFP 适用: 小鸡 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
alpha-Fetoprotein (AFP) ELISA KitAFP 适用: Pig Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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实验流程
- This assay employs an antibody specific for Anti-human AFP coated on a 96-well plate. Standards and samples are pipetted into the wells and AFP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human AFP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of AFP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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限制
- 仅限研究用
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注意事项
- protect your eyes
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储存条件
- 4 °C
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储存方法
- Please refer to the Manual
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- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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别名
- AFP (Alpha Fetoprotein)
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途径
- C21-Steroid Hormone Metabolic Process
抗原 See all alpha Fetoprotein (AFP) ELISA试剂盒
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