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Quick Overview for LTA ELISA 试剂盒 (ABIN5026953)
抗原
See all LTA ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- This assay employs an antibody specific for Anti-human TNFBeta coated on a 96-well plate.
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Analytical Method
- Quantitative
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灵敏度
- 9.4 pg/mL
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产品特性
- This immunoassay kit allows for the in vitro quantitative determination of Human TNFBeta concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL
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组件
- plate, standard, diluent, antibodies
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试剂未包括
- pipettes, tubes, reader, incubator
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Lymphotoxin-alpha (LTA) ELISA Kit
LTA 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 人 AA 35-205 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 犬 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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实验流程
- This assay employs an antibody specific for Anti-human TNFBeta coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFBeta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TNFBeta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFBeta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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限制
- 仅限研究用
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注意事项
- protect your eyes
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储存条件
- 4 °C
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储存方法
- Please refer to the Manual
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- LTA (Lymphotoxin-alpha (LTA))
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别名
- TNF Beta (Tumor Necrosis Factor Beta)
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原 See all LTA ELISA试剂盒
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