ALOX15 ELISA 试剂盒
Quick Overview for ALOX15 ELISA 试剂盒 (ABIN5026944)
抗原
See all ALOX15 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- This immunoassay kit allows for the in vitro quantitative determination of Human 15-LO concentrations in serum, plasma and other biological fluids. This kit is for Research Use Only. Not for use in diagnostic/therapeutics procedures.
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Analytical Method
- Quantitative
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灵敏度
- < 37.5 pg/mL
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组件
- plate, standard, diluent, antibodies
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试剂未包括
- pipettes, tubes, reader, incubator
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Arachidonate 15-Lipoxygenase (ALOX15) ELISA Kit
ALOX15 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Arachidonate 15-Lipoxygenase (ALOX15) ELISA KitALOX15 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Arachidonate 15-Lipoxygenase (ALOX15) ELISA KitALOX15 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Arachidonate 15-Lipoxygenase (ALOX15) ELISA KitALOX15 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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实验流程
- This assay employs an antibody specific for Anti-human 15-LO coated on a 96-well plate. Standards and samples are pipetted into the wells and 15-LO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human 15-LO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of 15-LO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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限制
- 仅限研究用
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注意事项
- protect your eyes
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储存条件
- 4 °C
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- ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))
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别名
- 15-LO (Arachidonate 15-Lipoxygenase)
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途径
- Regulation of Actin Filament Polymerization
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