IL-33 ELISA 试剂盒
Quick Overview for IL-33 ELISA 试剂盒 (ABIN5026939)
抗原
See all IL-33 (IL33) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-33 concentrations in serum, plasma and other biological fluids.
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Analytical Method
- Quantitative
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灵敏度
- < 9.4 pg/mL
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组件
- plate, standard, diluent, antibodies
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试剂未包括
- pipettes, tubes, reader, incubator
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Interleukin 33 (IL33) ELISA Kit
IL33 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 33 (IL33) ELISA KitIL33 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 33 (IL33) ELISA KitIL33 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 33 (IL33) ELISA KitIL33 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Urine
Interleukin 33 (IL33) ELISA KitIL33 适用: 犬 Colorimetric Sandwich ELISA 23.44 pg/mL - 1500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 33 (IL33) ELISA KitIL33 适用: 小鼠 AA 109-266 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 33 (IL33) ELISA KitIL33 适用: 人 AA 112-270 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 33 (IL33) ELISA KitIL33 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Interleukin 33 (IL33) ELISA KitIL33 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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实验流程
- This assay employs an antibody specific for Anti-humanIL-33 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-33 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-33 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-33 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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限制
- 仅限研究用
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注意事项
- protect your eyes
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储存条件
- 4 °C
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- IL-33 (IL33) (Interleukin 33 (IL33))
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别名
- IL-33 (Interleukin-33)
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途径
- Production of Molecular Mediator of Immune Response
抗原 See all IL-33 (IL33) ELISA试剂盒
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