IL29 ELISA 试剂盒
Quick Overview for IL29 ELISA 试剂盒 (ABIN5026936)
抗原
See all IL29 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 15.6 pg/mL
-
原理
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-29 concentrations in serum, plasma and other biological fluids.
-
Analytical Method
- Quantitative
-
灵敏度
- < 9.4 pg/mL
-
组件
- plate, standard, diluent, antibodies
-
试剂未包括
- pipettes, tubes, reader, incubator
-
-
-
-
样本量
- 100 μL
-
实验时间
- 3 - 4 h
-
板类型
- Pre-coated
-
实验流程
- This assay employs an antibody specific for Anti-humanIL-29 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-29present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylate-d anti-humanIL-29 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-29 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
限制
- 仅限研究用
-
-
-
注意事项
- protect your eyes
-
储存条件
- 4 °C
-
-
- IL29 (Interleukin 29 (IL29))
-
别名
- IL-29 (Interleukin 29)
抗原 See all IL29 ELISA试剂盒
-