IL-11 ELISA 试剂盒
Quick Overview for IL-11 ELISA 试剂盒 (ABIN5026925)
抗原
See all IL-11 (IL11) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-11 concentrations in serum, plasma and other biological fluids.
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Analytical Method
- Quantitative
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灵敏度
- 18.7 pg/mL
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组件
- plate, standard, diluent, antibodies
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试剂未包括
- pipettes, tubes, reader, incubator
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Interleukin 11 (IL11) ELISA Kit
IL11 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Interleukin 11 (IL11) ELISA KitIL11 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 11 (IL11) ELISA KitIL11 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 11 (IL11) ELISA KitIL11 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 11 (IL11) ELISA KitIL11 适用: 小鼠 AA 22-199 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 11 (IL11) ELISA KitIL11 适用: 人 AA 22-199 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 11 (IL11) ELISA KitIL11 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Interleukin 11 (IL11) ELISA KitIL11 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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实验流程
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IL-11antibody was pre-coated into 96-well plates. Biotin conjugated anti-humanIL-11detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-11amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human IL-11 can be calculated using the standard curve.
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限制
- 仅限研究用
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注意事项
- protect your eyes
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储存条件
- 4 °C
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- IL-11 (IL11) (Interleukin 11 (IL11))
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别名
- IL-11 (Interleukin 11)
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途径
- JAK/STAT Signaling, Negative Regulation of Hormone Secretion
抗原 See all IL-11 (IL11) ELISA试剂盒
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