Nivolumab Specific ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 Nivolumab Specific.
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产品编号 ABIN5012827
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Nivolumab Specific ELISA 试剂盒 (ABIN5012827)
抗原
Nivolumab Specific
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Plasma, Serum
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原理
- Enzyme immunoassay for the specific quantitative determination of free Nivolumab in serum and plasma
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Analytical Method
- Quantitative
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特异性
- There is no cross reaction with any other proteins present in native human serum. A screening test was performed with 48 different native human sera. All produced OD450/620 nm values (ranged from 0.043 to 0.100) less than the mean OD (0.150) of standard D (20 ng/mL). In addition, binding of Nivolumab to the solid phase is inhibited by recombinant human PD-1 protein in a concentration dependent manner. Therefore, the Nivolumab ELISA (mAb-Based) measures the biologically active free form of Nivolumab, i.e. not pre-occupied by human PD-1 antigen. No cross reaction was observed with sera spiked with the other therapeutic antibodies including Cetuximab, Aflibercept, Rituximab, Trastuzumab, Bevacizumab, Vedolizumab, Infliximab and Adalimumab at concentrations up to 200 μg/mL. All produced mean OD450/620 nm values (ranged from 0.024 to 0.030) less than standard D.
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组件
- plate, standards, assay buffer, conjugate, TMB, HCl, wash buffer
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试剂未包括
- normal lab equipment for performing ELISA assays
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样本量
- 20 μL
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实验时间
- 1.5 h
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板类型
- Pre-coated
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实验流程
- This ELISA is based on Nivolumab-specific mouse monoclonal antibody (catcher Ab, clone NY-11C3). Diluted standards and samples are incubated in the microtiter plate coated with IG-NY-11C3 mAb. After incubation, the wells are washed. A horseradish peroxidase (HRP)-conjugated anti-human IgG antibody is added and binds to the Fc part of Nivolumab. Following incubation, wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The color developed is proportional to the amount of Nivolumab in the sample or standard. Results of samples can be determined by using the standard curve.
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试剂准备
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Just the wash buffer has to be prepared by diluting the stock solution 1:20. All other reagents are ready to use.
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样品收集
- normal serum or plasma collection
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样品制备
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dilute the samples 1:20 with assay buffer
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结果分析
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The results are read from a standard curve.
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实验精密度
- < 10%
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限制
- 仅限研究用
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储存液
- Sodium azide
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储存条件
- 4 °C
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有效期
- 24 months
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- Nivolumab Specific
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背景
- The drug Nivolumab (Opdivo®) is a fully human IgG4 monoclonal antibody that binds specifically to programmed death-1 (PD-1), a negative regulatory receptor expressed by activated T and B lymphocytes. Binding of Nivolumab to the PD-1 receptor blocks its interaction with the ligands, PDL1 and PD-L2, thereby attenuating PD-1-mediated inhibition of the immune response, including the anti-tumor immune response. According to the prescribing information, Nivolumab at 3 mg/kg every 2 weeks has been shown to be safe and efficacious in the treatment of advanced melanoma, refractory non-small cell lung cancer (NSCLC), and advanced renal cell carcinoma, and it is being studied in patients with other solid tumors and hematologic malignancies. Following the recommended dose regimen (3 mg/kg every 2 week), the mean Cmin (trough) and Cmax concentrations of Nivolumab were reported to be 15 μg/mL and 68.8 μg/mL, respectively. Identification of biomarkers for (non-) response and risk factors for adverse drug reactions that might be related to serum drug levels and maintaining the effective concentration in order to potentially avoid some side effects with a reliable method might be beneficial.
抗原
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