Bevacizumab Specific ELISA 试剂盒
人, 猴, 小鼠 和 大鼠 Bevacizumab Specific ELISA Kit, Colorimetric is an assay for quantification of 人, 猴, 小鼠 和 大鼠 Bevacizumab Specific.
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产品编号 ABIN5012826
发货至:
中国
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Quick Overview for Bevacizumab Specific ELISA 试剂盒 (ABIN5012826)
抗原
Bevacizumab Specific
适用
人, 猴, 小鼠, 大鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Plasma, Serum
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原理
- Enzyme immunoassay for the specific quantitative determination of free Bevacizumab in serum and plasma
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Analytical Method
- Quantitative
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特异性
- There is no cross reaction with any other proteins present in native human serum. A screening test was performed with 48 different native human sera. All produced OD450/620 nm values (ranged from 0.022 to 0.042) less than the mean OD (0.132) of standard D (6 ng/mL). In addition, binding of Bevacizumab is inhibited by recombinant human VEGF-A in a concentration dependent manner. Therefore, the Bevacizumab ELISA (mAb-Based) measures the biologically active free form of Bevacizumab, i.e. not pre-occupied by VEGF. No cross reaction was observed with sera spiked with the other therapeutic antibodies including Infliximab, Adalimumab, Etanercept, Rituximab, Tocilizumab, Trastuzumab, Aflibercept and Golimumab at concentrations up to 2 mg/mL. All produced mean OD450/620 nm values ranged from 0.009 to 0.022. In addition, there is no cross reaction with Ranibizumab as well. Because when anti-human kappa monoclonal antibody was used as the conjugate instead, Ranibizumab did not bind to MAY-2B5 mAb-coated plate.
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组件
- plate, standards, assay buffer, conjugate, TMB, HCl, wash buffer
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试剂未包括
- normal lab equipment for performing ELISA assays
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样本量
- 20 μL
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实验时间
- 1.5 h
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板类型
- Pre-coated
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实验流程
- This ELISA is based on Bevacizumab-specific mouse monoclonal antibody (catcher Ab, clone MAY-2B5). Standards and diluted samples are incubated in the microtiter plate coated with IG-MAY-2B5 mAb. After incubation, the wells are washed. A horseradish peroxidase (HRP)-conjugated anti-human IgG monoclonal antibody is added and binds to the Fc part of Bevacizumab. Following incubation, wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The color developed is proportional to the amount of Bevacizumab in the sample or standard. Results of samples can be determined by using the standard curve. Binding of Bevacizumab to the solid phase, pre-coated with MAY-2B5, is inhibited by human VEGF-A in a concentration dependent manner. Therefore, the Bevacizumab ELISA (mAb-Based) measures the free form of Bevacizumab.
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试剂准备
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Just the wash buffer has to be prepared by diluting the stock solution 1:20. All other reagents are ready to use.
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样品收集
- normal serum or plasma collection
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样品制备
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dilute the samples 1:20 with assay buffer
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结果分析
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The results are read from a standard curve.
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实验精密度
- < 10%
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限制
- 仅限研究用
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储存液
- Sodium azide
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储存条件
- 4 °C
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有效期
- 24 months
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- Bevacizumab Specific
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背景
- The drug Bevacizumab (trade name Avastin®) is a recombinant human IgG1: monoclonal antibody specific for all human vascular endothelial growth factor-A (VEGF-A) isoforms and it has been approved by the FDA as a first-line treatment for metastatic colorectal cancer in combination with chemotherapy. Furthermore, VEGF is implicated in intraocular neovascularization associated with diabetic retinopathy and age-related macular degeneration. According to the data indicated in the prescribing information and the literature, serum/plasma Bevacizumab concentration of patients, under the treatment of Bevacizumab as an intravenous (IV) infusion at various doses of 5-15 mg/kg every 2-3 weeks, reported to be ranging from 10 μg/mL as the Cmin to 200 μg/mL as the Cmax. Serum through levels might be related to predict some clinical outcome during maintenance therapy. It was also possible that the surveillance of circulating concentration during maintenance therapy represents a direct and/or indirect factor for some other side effects. In this context, identification of biomarkers for (non-) response and risk factors for adverse drug reactions that might be related to serum drug levels and maintaining the effective concentration in order to potentially avoid some side effects with a reliable method might be beneficial.
抗原
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