11,12-Dihydroxyeicosatrienoic Acids (11,12-DHETs) ELISA 试剂盒
适用: 人
Colorimetric
Competition ELISA
1.563-100 ng/mL
Plasma, Serum
1 image
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- 抗原
- 11,12-Dihydroxyeicosatrienoic Acids (11,12-DHETs)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 1.563-100 ng/mL
- 最低检测浓度
- 1.563 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a competitive enzyme immunoassay for in vitro quantitative measurement of 11,12-DHETs in human serum, plasma and other biological fluids
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This kit recognizes natural and recombinant Human 11,12-DHETs. No significant cross-reactivity or interference between Human 11,12-DHETs and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between Human 11,12-DHETs and all the analogues.
- 灵敏度
- 0.938 ng/mL
- 组件
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Micro ELISA Plate(Dismountable)
Reference Standard
Concentrated Biotinylated Detection Ab (100×)
Concentrated HRP Conjugate (100×)
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solution
Plate Sealer
Product Description
Certificate of Analysis - 试剂未包括
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Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer
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- 说明
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Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).
Information on reagents:
Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.
Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration. - 样本量
- 50 µL
- 板类型
- Pre-coated
- 实验流程
- This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 11,12-DHETs. During the reaction, 11,12-DHETs in the sample or standard competes with a fixed amount of 11,12-DHETs on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 11,12-DHETs. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 11,12-DHETs in the samples is then determined by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
- 限制
- 仅限研究用
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- 注意事项
- All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
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- 抗原
- 11,12-Dihydroxyeicosatrienoic Acids (11,12-DHETs)
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