VCAM1 ELISA 试剂盒
Quick Overview for VCAM1 ELISA 试剂盒 (ABIN4987154)
抗原
See all VCAM1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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Analytical Method
- Quantitative
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特异性
- Natural and recombinant Human VCAM-1 Ligand
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灵敏度
- 31 pg/mL
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试剂未包括
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- Microplate reader.
- Pipettes and pipette tips.
- EP tube Deionized or distilled water.
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Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit
VCAM1 适用: 人 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 小鼠 Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 小鼠 Colorimetric Sandwich ELISA 39.062-2500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 大鼠 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 兔 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 人 AA 25-698 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 犬 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 猴 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Detection Wavelength: 450 nm
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样本量
- 20 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
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别名
- VCAM-1
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背景
- Human Vascular Cell Adhesion Molecule-1 (VCAM-1) is a 100 - 110 kDa, 715 amino acid (aa) type I transmembrane glycoprotein typically characterized by the presence of seven C2-type immunoglobulin (Ig) domains (1 - 3). Its extracellular region is 674 aa in length, followed by a 22 aa transmembrane segment and a 19 aa cytoplasmic tail (1, 2). In the extracellular region, there are multiple N-linked glycosylation sites (the predicted molecular weight is 80 kDa), and each C2 domain is closed by a disulfide bridge. There is considerable interspecies VCAM-1 homology, with mouse and rat VCAM-1 showing approximately 75 % aa identity to human VCAM-1 (2 - 4). Notably, the short 19 aa cytoplasmic tail is absolutely conserved, mouse to human to rat (4). Cells expressing mouse VCAM-1 bind both mouse and human leukocytes, and this reflects their high degree of aa identity (4). A number of variants of VCAM-1 are known to occur, all of which are likely the result of alternate gene splicing. In particular, a human six Ig domain molecule is known (1), and in rabbits, an eight Ig domain form has been identified (2). There is also a three-C2 domain, 43 kDa GPI-linked form of VCAM-1 (5, 6). Although it binds known VCAM-1 ligands (or co-receptors), its function is unclear. Cells known to express VCAM-1 include neurons (7), endothelial cells (8), smooth muscle cells (9), fibroblasts (10) and macrophages (11). Soluble VCAM-1 has been identified in culture supernates (12), blood (13 - 15), and cerebrospinal fluid (15, 16). In vitro, basal levels of VCAM-1 shedding by unstimulated NIH3T3 cells appear to partially require metalloproteinase activity, while PMA-induced shedding is dependent upon the proteolytic activity of TACE/ADAM17 (12).Functionally, VCAM-1 binds to both a4b1 (VLA-4) and a4b7 (LPAM-1) integrins (17, 18). These integrins (or VCAM-1 ligands) are expressed on a variety of cells, with VLA-4 found on all leukocytes with the exception of neutrophils (17, 19, 20). Because of this, VCAM-1/VCAM-1 ligand interactions are undoubtedly key events in the rate and timing of leukocyte extravasation(3). Other roles proposed for VCAM-1 include the regulation of osteoclastogenesis via a cell-to-cell contact mechanism (22) and the induction of sickle cell adherence to vascular endothelial cells during hypoxemia (23).
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途径
- Carbohydrate Homeostasis
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