Soluble E-Selectin ELISA 试剂盒
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Quick Overview for Soluble E-Selectin ELISA 试剂盒 (ABIN4987075)
抗原
See all Soluble E-Selectin (sE-Selectin) products适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 780 pg/mL
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Analytical Method
- Quantitative
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特异性
- Natural and recombinant Human sE-selectin Ligand
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灵敏度
- 200 pg/mL
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试剂未包括
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- Microplate reader.
- Pipettes and pipette tips.
- EP tube Deionized or distilled water.
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Selectin E (Soluble) (sE-Selectin) ELISA Kit
sE-Selectin 适用: 小鼠 AA 22-557 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (citrate), Plasma (heparin), Serum
Selectin E (Soluble) (sE-Selectin) ELISA KitsE-Selectin 适用: 大鼠 AA 22-494 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (citrate), Plasma (heparin), Serum
Selectin E (Soluble) (sE-Selectin) ELISA KitsE-Selectin 适用: 大鼠 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Selectin E (Soluble) (sE-Selectin) ELISA KitsE-Selectin 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (heparin - citrate), Serum
Selectin E (Soluble) (sE-Selectin) ELISA KitsE-Selectin 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (heparin - citrate), Serum
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应用备注
- Detection Wavelength: 450 nm
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样本量
- 20 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))
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别名
- sE-Selectin
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背景
- E-Selectin (also known as Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, or CD62E) is a 115 kDa, type I transmembrane glycoprotein expressed only on endothelial cells and only after activation by inflammatory cytokines (IL-1β or TNF-α) or endotoxins (1-4). Expression is transitory, reaching a maximum within about 6 hours of stimulation and then declining with shedding of soluble E-Selectin (1-5). Cell-surface E-Selectin is a mediator of the rolling attachment of leukocytes to the endothelium, an essential step in extravasation of leukocytes at the site of inflammation (1-6) thereby playing a key role in localized inflammatory response. E-Selectin is believed to be particularly important in inflammation involving the skin (4).The extracellular part of E-Selectin includes a calcium-dependent C2-type lectin domain, an epidermal growth factor (EGF) domain, and six repeats of a complement-regulatory-protein-like sequence (1-4). E-Selectin binds sialyl Lewis X (sLex), a sialic acid-galactose-N-acetylglucosamine-fructose tetrasaccharide, but the actual recognition is thought to be for a specific presentation of those glycosyl units in a precise three-dimensional configuration on a specific glycoprotein rather than for that particular carbohydrate (4).Soluble E-Selectin (sE-Selectin) is found in the blood of healthy individuals (7, 8), probably arising from proteolytic cleavage of the surface-expressed molecule (5, 9). Elevated levels of sE-Selectin in serum have been reported in a variety of pathological conditions (8, 10-14). Although it might be anticipated that sE-Selectin would suppress leukocyte migration by competing with surface-associated E-Selectin, it may actually activate neutrophils and act as a pro-inflammatory agent (15).
抗原 See all Soluble E-Selectin (sE-Selectin) products
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