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IL-5 ELISA 试剂盒

This 小鼠 IL-5 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 IL-5.
产品编号 ABIN4986960
发货至: 中国

Quick Overview for IL-5 ELISA 试剂盒 (ABIN4986960)

抗原

See all IL-5 (IL5) ELISA试剂盒
IL-5 (IL5) (Interleukin 5 (IL5))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.02-1.0 ng/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)
  • 最低检测浓度

    0.02 ng/mL

    Analytical Method

    Quantitative

    特异性

    Natural and recombinant Mouse IL-5 Ligand

    灵敏度

    7 pg/mL

    试剂未包括

    • Microplate reader.
    • Pipettes and pipette tips.
    • EP tube Deionized or distilled water.
  • 应用备注

    Detection Wavelength: 450 nm

    样本量

    20 μL

    实验时间

    3 h

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C
  • 抗原 See all IL-5 (IL5) ELISA试剂盒

    IL-5 (IL5) (Interleukin 5 (IL5))

    别名

    IL-5

    背景

    Mouse interleukin 5 (mIL-5), also known as T-cell replacing factor, B-cell growth factor II, eosinophil differentiation factor and eosinophil colony stimulating factor, is a pleiotropic cytokine produced primarily by T cells (1-4). It supports the proliferation and differentiation of mouse, but not human, B cells, and enhances IgM, IgG1, IgA, and IgE secretion. IL-5 chemoattracts and enhances the survival and the effector functions of mature eosinophils and synergizes with various colony stimulating factors to increase eosinophil progenitor production and eosinophil expansion. Because of its diverse effects on eosinophils, IL-5 is strongly implicated in the pathogenesis of asthma and other hypereosinophilic inflammatory conditions (4-6). Mouse IL-5 cDNA encodes a 133 amino acid (aa) residue precursor protein containing a hydrophobic signal peptide that is cleaved to yield a 113 aa residue mature protein. Native mouse IL-5 is a disulfide-linked homodimeric 40-45 kDa glycoprotein with N- and O-linked carbohydrate chains. Mature human IL-5 is approximately 70 % identical at the amino acid level to mouse IL-5. Whereas mouse and human IL-5 are equally active on human cell lines, human IL-5 is much less active than mouse IL-5 in mouse cell assays (1-3). IL-5 is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes.

    途径

    JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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