IL2R ELISA 试剂盒
This 人 IL2R ELISA Kit specifically detects IL2R
in Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate) 和 Plasma (EDTA).
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产品编号 ABIN4986952
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for IL2R ELISA 试剂盒 (ABIN4986952)
抗原
See all IL2R ELISA试剂盒
IL2R
(Interleukin 2 Receptor (IL2R))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
31.25-2000 pg/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)
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最低检测浓度
- 31.25 pg/mL
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Analytical Method
- Quantitative
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特异性
- Natural and recombinant Human IL-2R Ligand
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灵敏度
- 15 pg/mL
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试剂未包括
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- Microplate reader.
- Pipettes and pipette tips.
- EP tube Deionized or distilled water.
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Interleukin 2 Receptor (IL2R) ELISA Kit
IL2R 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor (IL2R) ELISA KitIL2R 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Detection Wavelength: 450 nm
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样本量
- 20 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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- IL2R (Interleukin 2 Receptor (IL2R))
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别名
- IL-2R
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背景
- The biological activities of IL-2 are mediated by its binding to a multi-molecular cellular receptor complex. For several years the receptor was thought to consist of two glycoprotein chains, an alpha chain (IL-2 Rα) and a beta chain (IL-2 Rβ) (1 - 3), which acted together to form a high affinity receptor that transduced the IL-2 signal. IL-2 Rα (also known as Tac antigen and as CD25) is a 55 kDa transmembrane glycoprotein composed of 351 amino acids with only 13 located on the cytoplasmic side of the membrane (4 - 6). The second chain of the complex was cloned in 1989 (7), and is a transmembrane glycoprotein of 575 amino acids (75 kDa), 286 of which are located cytoplasmically and clearly participate in signal transduction (8, 9). Eventually it was discovered that a third chain, IL-2 Rγ, was necessary for high affinity binding, ligand internalization and signalling. Constitutively expressed on many lymphoid cells, it had been overlooked partly because it has no affinity for IL-2 except when IL-2 Rβ is present (7, 10, 11). When cloned, the gene was found to code for a 64 kDa transmembrane protein of 347 amino acids, 84 of which are cytoplasmic (12). Both IL-2 Rβ and IL-2 Rγ are members of the hematopoietin receptor superfamily, whereas IL-2 Rα is related only to the IL-15 R αchain (13 - 15).A model of the IL-2 receptor complex (3, 9, 16 - 21) would describe the high affinity receptor as an αβγ trimer, in which all three chains are in contact with the ligand. Alone, IL-2 Rα binds IL-2 with low affinity, but is unable to transduce a signal. The αβ combination will bind IL-2 with intermediate affinity, but still will not transduce a signal. A βγ complex has intermediate affinity and is capable of signalling if the IL-2 concentration is relatively high. Regardless of many subtleties that determine the affinity of the ligand for the extracellular portions of the receptor components (22 - 24), signalling will ensue if the βand γcytoplasmic domains are brought into close proximity (25 - 27).A soluble form of IL-2 Rα appears in serum, concomitant with its increased expression on cells (18, 28, 29). There are reports of a soluble form of IL-2 Rβ as well (28, 30). The function of the soluble IL-2 Rα is unclear, since it would be expected to be a poor inhibitor of IL-2 because of its low binding affinity. In any case, increased levels of the soluble IL-2 Rα in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Results of a number of studies suggest a correlation of levels of IL-2 sRα in serum with the onset of rejection episodes in allograft recipients (18, 31 - 33), with activity of autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosis (SLE) (34) and with the course of some leukemias and lymphomas (35 - 40).
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