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IL-17 ELISA 试剂盒

This 人 IL-17 ELISA Kit specifically detects IL-17 in Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate) 和 Plasma (EDTA).
产品编号 ABIN4986934
发货至: 中国
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Quick Overview for IL-17 ELISA 试剂盒 (ABIN4986934)

抗原

See all IL-17 (IL17) ELISA试剂盒
IL-17 (IL17) (Interleukin 17 (IL17))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.625-1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)
  • 最低检测浓度

    15.625 pg/mL

    Analytical Method

    Quantitative

    特异性

    Natural and recombinant Human IL-17 Ligand

    灵敏度

    7 pg/mL

    试剂未包括

    • Microplate reader.
    • Pipettes and pipette tips.
    • EP tube Deionized or distilled water.
  • 应用备注

    Detection Wavelength: 450 nm

    样本量

    20 μL

    实验时间

    3 h

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C
  • 抗原 See all IL-17 (IL17) ELISA试剂盒

    IL-17 (IL17) (Interleukin 17 (IL17))

    别名

    IL-17

    背景

    IL-17, originally identified as mouse cytotoxic T lymphocyte-associated antigen-8 (CTLA-8) (1), is produced by activated T lymphocytes, primarily by memory T cells (1-4). IL-17 appears to mediate communication between the immune system and the hematopoietic system.IL-17 is a disulfide-linked homodimer (2, 4). Each polypeptide has 155 amino acid (aa) residues (predicted mass = 17.5 kDa), including a 19 aa residue hydrophobic leader sequence (2). There are six cysteines plus one potential N-linked glycosylation site, which is variably glycosylated, at least with recombinant proteins (2, 4). The aa sequence of human IL-17 is 63 % and 58 % identical to mouse and rat IL-17 and 72 % identical to the thirteenth ORF of Herpesvirus saimiri (2, 4). There is at least some species specificity for in vitro action on bone-marrow stromal cells (3).IL-17 mediation of T cell communication with the hematopoietic system is suggested by two observations. T cell-derived IL-17 induces fibroblasts to produce IL-6, IL-8, ICAM-1 and G-CSF, apparently by an NF-?B-mediated mechanism (5). IL-6 in turn promotes development of granulocyte/macrophage colonies, and G-CSF directs development of neutrophils (4, 6-9). IL-17 also enhances proliferation of partially activated T cells (5) and upregulates nitric oxide (NO) production in osteoarthritic cartilage (10).
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