IL-13 ELISA 试剂盒
Quick Overview for IL-13 ELISA 试剂盒 (ABIN4986932)
抗原
See all IL-13 (IL13) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 3.125 pg/mL
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Analytical Method
- Quantitative
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特异性
- Natural and recombinant Human IL-13 Ligand
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灵敏度
- 1 pg/mL
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试剂未包括
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- Microplate reader.
- Pipettes and pipette tips.
- EP tube Deionized or distilled water.
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Interleukin 13 (IL13) ELISA Kit
IL13 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 适用: Pig Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 适用: 马 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 适用: 豚鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 适用: 小鸡 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 适用: 人 AA 21-132 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Detection Wavelength: 450 nm
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样本量
- 20 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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- IL-13 (IL13) (Interleukin 13 (IL13))
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别名
- IL-13
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背景
- Interleukin-13 (IL-13) is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis (1-3). It is secreted by Th1, Th2, Th17, NK, and mast cells, visceral smooth muscle cells, eosinophils, and basophils (2, 4). This pattern is similar to the expression of Interleukin-4 (IL-4) but also includes subsets of Th1 and Th17 cells that do not secrete IL-4 (5). IL-13 circulates as a monomer and has two internal disulfide bonds that contribute to its bundled four α-helix configuration (6, 7). Mature human IL-13 shares approximately 58 % amino acid sequence identity with mouse and rat IL-13. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (8, 9). IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. On B cells, it promotes cellular activation, immunoglobulin class switching to IgE, and the upregulation of CD23/Fcε RII. Polymorphisms and upregulation of IL-13 are associated with atopy, asthma, airway hyperresponsiveness, and tissue fibrosis (1, 10).The biological effects of IL-13 and IL-4 are closely related due in part to a shared receptor system. IL-13 binds with low affinity to the transmembrane IL-13 Rα1 which then forms a signaling complex with the transmembrane IL-4 Rα (11-13). This high affinity receptor complex also functions as the type 2 IL-4 receptor (11, 12). Soluble forms of IL-4 Rα are expressed which retain ligand binding properties and inhibit IL-4 bioactivity (14, 15). IL-4 Rα also associates with the common gamma chain (γc) to form the type 1 IL-4 receptor complex (16, 17).Additionally, IL-13 binds with high affinity to IL-13 Rα2 which is expressed as cell surface and soluble forms (18-20). IL-13 Rα2 functions as a decoy receptor by preventing IL-13 from signaling through the IL-13 Rα1/IL-4 Rα complex (21, 22). It also inhibits responsiveness to IL-4 by blocking signaling through IL-4-occupied IL-13 Rα1/IL-4 Rα receptor complexes (22, 23). IL-13 Rα2 is upregulated during Th2-biased immune responses and limits inflammatory tissue damage (20, 24, 25). Aside from its decoy function, IL-13 Rα2 can signal in response to IL-13 to directly promote tumor cell invasiveness and the development of tissue fibrosis (26-28).
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途径
- JAK/STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
抗原 See all IL-13 (IL13) ELISA试剂盒
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