IgE ELISA 试剂盒
Quick Overview for IgE ELISA 试剂盒 (ABIN4986922)
抗原
See all IgE ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.31 ng/mL
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Analytical Method
- Quantitative
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特异性
- Natural and recombinant Mouse IgE Ligand
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灵敏度
- 156 pg/mL
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试剂未包括
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- Microplate reader.
- Pipettes and pipette tips.
- EP tube Deionized or distilled water.
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IgE ELISA Kit
适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
IgE ELISA Kit适用: 兔 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
IgE ELISA Kit适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgE ELISA Kit适用: 人 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Urine
IgE ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 125 ng/mL - 8000 ng/mL Cell Culture Supernatant, Plasma, Serum
IgE ELISA Kit适用: Cow Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
IgE ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgE ELISA Kit适用: 犬 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgE ELISA Kit适用: Cat Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgE ELISA Kit适用: 犬 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Detection Wavelength: 450 nm
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样本量
- 20 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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- IgE
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背景
- There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgG, and IgM. In mice, the IgG class is further divided into four subclasses: IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3.The general immunoglobulin structure is described as two heavy and two light chain (H2L2) polypeptides linked together by disulfide bridges of cysteine residues. Mouse IgE, also known as reaginic antibody, exists in serum as a 4-chain monomer of 185-200 kDa. Like IgM, the IgE monomers contain a fourth constant region domain. Serum concentrations of IgE are reported to be >10 μg/mL. IgE is cyolytic for mast cells, where it binds to the Fc?RI receptor and is able to mediate the response of granular and lipid mediators of inflammation, including the PCA reaction. The IgE class switch is induced by IL-4 as part of the Th2 response.
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