Triiodothyronine T3 ELISA 试剂盒
Quick Overview for Triiodothyronine T3 ELISA 试剂盒 (ABIN4973540)
抗原
See all Triiodothyronine T3 (T3) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
质量等级
-
-
最低检测浓度
- 123.5 pg/mL
-
原理
- Low Sample Volume Triiodothyronine (T3) ELISA Kit
-
Analytical Method
- Quantitative
-
灵敏度
- < 51.7 pg/mL
-
组件
-
The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- Pre-coated 96-Well Microplate
- Standard
- Standard Diluent Buffer
- Wash Buffer
- Detection Reagent A
- Detection Reagent B
- Diluent A
- Diluent B
- TMB Substrate
- Stop Solution
- Plate Sealer
-
试剂未包括
-
- 37 °C incubator
- Multi and single channel pipettes and sterile pipette tips
- Squirt bottle or automated microplate washer
- 1.5 mL tubes
- Distilled water
- Absorbent filter papers
- 100 mL and 1 liter graduated cylinders
- Microplate reader (wavelength: 450 nm)
- ELISA Shaker
-
-
Triiodothyronine T3 (T3) ELISA Kit
T3 适用: Various Species Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Plasma, Serum
Triiodothyronine T3 (T3) ELISA KitT3 适用: Various Species Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Triiodothyronine T3 (T3) ELISA KitT3 适用: 大鼠 Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Triiodothyronine T3 (T3) ELISA KitT3 适用: Pig Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Triiodothyronine T3 (T3) ELISA KitT3 适用: Cow Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Triiodothyronine T3 (T3) ELISA KitT3 适用: 小鸡 Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Triiodothyronine T3 (T3) ELISA KitSmall Sample T3 适用: Various Species Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Plasma, Serum
-
-
应用备注
- Optimal dilutions/concentrations should be determined by the end user.
-
说明
-
The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
-
样本量
- 25 µL
-
板类型
- Pre-coated
-
试剂准备
-
This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.
- 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.
- 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.
-
实验流程
-
This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- Equilibrate the kit components and samples to room temperature (18 - 25 °C) before use. It is recommended to plot a standard curve for each test.
- 1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample at least in duplicate.
- 2. Add 25 μL of each standard, control and sample into the appropriate wells. Seal the plate with a cover and incubate for 1 h at 37 °C.
- 3. Remove the cover and discard the liquid.
- 4. Add 25 μL of the detection Reagent A working solution to each well. Seal the plate with a cover and incubate for 1 h at 37 °C.
- 5. Remove the cover and discard the solution. Wash the plate 3 times with 1X Wash Buffer.
- 6. Add 25 μL of Detection Reagent B working solution into each well, seal and incubate at 37 °C for 30 min.
- 7. Discard the solution and wash the plate 5 times with wash buffer as explained in previous step.
- 8. Aliquot 25 μL of TMB Substrate into each well. Seal the plate with a cover and incubate at 37 °C for 10-20 min. Avoid exposure to light. The incubation time is for reference use only, the optimal time should be determined by end user. Do not exceed 30 min.
- 9. Add 20 μL of Stop Solution to each well. Read at 450 nm immediately.
-
结果分析
-
This assay is competitive, therefore there is an inverse correlation between T3 concentration in the sample and the absorbance measured. Create a graph with the log of the standard concentration (y-axis) and average absorbance measured (x-axis). Apply a best fit trendline through the standard points. The T3 concentration of the samples can be interpolated from the standard curve.
-
实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Triiodothyronine were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Triiodothyronine were tested on 3 different plates, 8 replicates in each plate.
CV (%) = (Standard Deviation / mean) x 100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
储存方法
- Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
-
有效期
- 6 months
-
-
- Triiodothyronine T3 (T3)
-
别名
- Triiodothyronine
-
物质类
- Amino Acid
抗原 See all Triiodothyronine T3 (T3) ELISA试剂盒
-
