Gastrin-Releasing Peptide ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for Gastrin-Releasing Peptide ELISA 试剂盒 (ABIN4889773)
抗原
See all Gastrin-Releasing Peptide (GRP) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 0.1 ng/mL
-
原理
- Human GRP EIA Kit optimized for serum, plasma, and cell culture supernatants. Competition-based ELISA
-
Analytical Method
- Quantitative
-
特异性
- This EIA kit is designed to detect human Gastrin-releasing peptide.
-
灵敏度
- 0.4 ng/mL
-
产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
-
试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
-
-
Gastrin-Releasing Peptide (GRP) ELISA Kit
GRP 适用: 人 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Gastrin-Releasing Peptide (GRP) ELISA KitGRP 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Gastrin-Releasing Peptide (GRP) ELISA KitGRP 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Serum, Tissue Homogenate
Gastrin-Releasing Peptide (GRP) ELISA KitGRP 适用: 小鼠 Colorimetric Sandwich ELISA 39.063-2500 pg/mL Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Suggested Plasma Dilution: Human: 2X
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
-
试剂准备
-
Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 8x / Rat: 2x
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
-
有效期
- 6 months
-
-
- Gastrin-Releasing Peptide (GRP)
-
别名
- GRP
-
背景
-
Gene Symbols: GRP
Background: Gastrin-releasing peptide (GRP), is a 27-amino acid neuropeptide. Gastrin-releasing peptide stimulates the release of Gastrin from the G cells of the stomach, and regulates gastric acid secretion and enteric motor function. Gastrin-releasing peptide is also an important growth-modulating factor in developing lung epithelium. It has been used as a tumor marker in the diagnosis of small-cell lung carcinoma, since it is known to be produced by these cancer cells. GRP is also involved in the biology of the circadian system, playing a role in the signaling of light to the master circadian oscillator in the suprachiasmatic nuclei of the hypothalamus. Furthermore, GRP seems to mediate certain aspects of stress.
Protein Name (Synonyms): Gastrin-releasing peptide (GRP) [Cleaved into: Neuromedin-C (GRP-10)]
-
基因ID
- 2922
-
UniProt
- P07492
-
途径
- Peptide Hormone Metabolism, Hormone Activity
抗原 See all Gastrin-Releasing Peptide (GRP) ELISA试剂盒
-
