ERK1, JNK, P38 ELISA 试剂盒
The 人, 小鼠 和 大鼠 ERK1, JNK, P38 ELISA Kit (ABIN4886189) is a Colorimetric ELISA Kit designed to quantify 人, 小鼠 和 大鼠 ERK1, JNK, P38. This ELISA Kit has been cited in 2 publications.
(2 references)
产品编号 ABIN4886189
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for ERK1, JNK, P38 ELISA 试剂盒 (ABIN4886189)
抗原
ERK1, JNK, P38
抗原表位
pThr180, pThr183, pThr202, pTyr182, pTyr185, pTyr204
适用
人, 小鼠, 大鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Cell Lysate, Tissue Lysate
-
-
原理
- Phospho-ERK/JNK/P38? ELISA Kit. This assay semi-quantitatively measures phophorylated ERK/JNK/P38? in lysate samples.
-
Analytical Method
- Semi-Quantitative
-
特异性
- The antibody pair provided in this kit recognizes ERK1/2 (T202/Y204), JNK (T183/Y185), P38α (T180/Y182), Total ERK1/2, JNK and P38α in Human, Mouse and Rat Cell Lysates.
-
产品特性
-
- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
-
试剂未包括
-
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
-
-
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
-
有效期
- 6 months
-
-
-
: "Ethanol impairs intestinal barrier function in humans through mitogen activated protein kinase signaling: a combined in vivo and in vitro approach." in: PLoS ONE, Vol. 9, Issue 9, pp. e107421, (2015) (PubMed).
: "Reno-protective effect of NECA in diabetic nephropathy: implication of IL-18 and ICAM-1." in: European cytokine network, Vol. 23, Issue 3, pp. 78-86, (2012) (PubMed).
-
-
- ERK1, JNK, P38
-
别名
- ERK1, JNK , P38
抗原
-
You are here:
