Caveolin-1 ELISA 试剂盒
-
- 抗原 See all Caveolin-1 (CAV1) ELISA试剂盒
- Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))
- 抗原表位
- pTyr14, total
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-Caveolin-1 (Tyr14) and Total Caveolin-1 ELISA Kit. This assay semi-quantitatively measures phophorylated Caveolin-1 (Tyr14) and Total Caveolin-1 in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human Caveolin-1 phosphorylated at site Tyrosine-14 as well as total Caveolin-1.
- 产品特性
-
- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
-
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Featured
- Discover our best selling CAV1 ELISA Kit
- Top Product
- Discover our top product CAV1 ELISA Kit
-
Caveolin 1, Caveolae Protein, 22kDa (CAV1) ELISA Kit
CAV1 适用: 人 Colorimetric Sandwich ELISA 0.24 ng/mL - 15 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Caveolin 1, Caveolae Protein, 22kDa (CAV1) ELISA KitCAV1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Caveolin 1, Caveolae Protein, 22kDa (CAV1) ELISA KitCAV1 适用: 犬 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Caveolin 1, Caveolae Protein, 22kDa (CAV1) ELISA KitCAV1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Caveolin 1, Caveolae Protein, 22kDa (CAV1) ELISA KitCAV1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Caveolin 1, Caveolae Protein, 22kDa (CAV1) ELISA KitCAV1 适用: 小鸡 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
-
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
-
- 抗原 See all Caveolin-1 (CAV1) ELISA试剂盒
- Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))
- 别名
- Caveolin-1 (CAV1 产品)
- 别名
- fc07c04 ELISA Kit, wu:fc07c04 ELISA Kit, Cav-1 ELISA Kit, bscl3 ELISA Kit, cav1 ELISA Kit, cav1a ELISA Kit, cgl3 ELISA Kit, vip21 ELISA Kit, cav1b ELISA Kit, caveolin-1 ELISA Kit, cav ELISA Kit, mstp085 ELISA Kit, Caveolin-1 ELISA Kit, CAV1 ELISA Kit, cav-1 ELISA Kit, Cav ELISA Kit, caveolin 1 ELISA Kit, BSCL3 ELISA Kit, CGL3 ELISA Kit, MSTP085 ELISA Kit, PPH3 ELISA Kit, VIP21 ELISA Kit, caveolin 1 ELISA Kit, caveolin 1 L homeolog ELISA Kit, caveolin 1 S homeolog ELISA Kit, caveolin 1, caveolae protein ELISA Kit, caveolin 1, caveolae protein, 22kDa ELISA Kit, Caveolin ELISA Kit, cav1 ELISA Kit, cav1.L ELISA Kit, cav1.S ELISA Kit, CAV1 ELISA Kit, Cav1 ELISA Kit, cav-1 ELISA Kit
- 基因ID
- 857
- UniProt
- Q03135
- 途径
- Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals
-
