电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

VCAM1 ELISA 试剂盒

VCAM1 适用: 小鼠 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN4885815
发货至: 中国
  • 抗原 See all VCAM1 ELISA试剂盒
    VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
    适用
    • 9
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Mouse VCAM-1 (CD106) SpeedELISA Kit.
    品牌
    SpeedELISA
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    产品特性
    • Compressed workflow with only one wash step and three incubation steps
    • 3 hours total processing time
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Streptavidin-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Capture Antibody
    • HRP Conjugated Detection Antibody
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling VCAM1 ELISA Kit
    Top Product
    Discover our top product VCAM1 ELISA Kit
  • 样本量
    100 μL
    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 70 μL of the Antibody Cocktail to each well.
    3. Incubate 10 minutes at RT.
    4. Add 50 μL of sample or standard to each well.
    5. Incubate 2 h at RT.
    6. Wash Wells
    7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    8. Incubate 30 min at RT.
    9. Add 50 μL of Stop Solution to each well.
    10. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
    Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
    有效期
    6 months
  • 抗原 See all VCAM1 ELISA试剂盒
    VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
    别名
    VCAM-1 (VCAM1 产品)
    别名
    CD106 ELISA Kit, INCAM-100 ELISA Kit, Vcam-1 ELISA Kit, VCAM1B ELISA Kit, fi76d06 ELISA Kit, wu:fi76d06 ELISA Kit, zgc:158875 ELISA Kit, VCAM1 ELISA Kit, vascular cell adhesion molecule 1 ELISA Kit, vascular cell adhesion molecule 1b ELISA Kit, VCAM1 ELISA Kit, Vcam1 ELISA Kit, vcam1b ELISA Kit, VCAM-1 ELISA Kit
    基因ID
    22329
    UniProt
    P29533
    途径
    Carbohydrate Homeostasis
You are here:
客服