IL17F ELISA 试剂盒
(2 references)Our Local Distributor
北京 101111
Quick Overview for IL17F ELISA 试剂盒 (ABIN4885567)
抗原
See all IL17F ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- Custom Mouse Interleukin 17F ELISA Kit.
-
Analytical Method
- Quantitative
-
特异性
- The antibody pair provided in this kit recognizes Mouse Interleukin 17F.
-
产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
Interleukin 17F (IL17F) ELISA Kit
IL17F 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 17F (IL17F) ELISA KitIL17F 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 17F (IL17F) ELISA KitIL17F 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 17F (IL17F) ELISA KitIL17F 适用: 小鼠 AA 21-153 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 17F (IL17F) ELISA KitIL17F 适用: 大鼠 AA 19-153 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 17F (IL17F) ELISA KitIL17F 适用: 人 Colorimetric Sandwich ELISA 32-2000 pg/mL Cell Lysate, Plasma, Serum
-
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
-
有效期
- 6 months
-
-
-
: "Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-?b, STAT3 and pro-inflammatory mediators and enhancement of I?B? and anti-inflammatory ..." in: Inflammation, Vol. 38, Issue 2, pp. 846-57, (2015) (PubMed).
: "Serum levels of IL-10, IL-17F and IL-33 in patients with asthma: a case-control study." in: The Journal of asthma : official journal of the Association for the Care of Asthma, Vol. 51, Issue 10, pp. 1004-13, (2014) (PubMed).
-
: "Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-?b, STAT3 and pro-inflammatory mediators and enhancement of I?B? and anti-inflammatory ..." in: Inflammation, Vol. 38, Issue 2, pp. 846-57, (2015) (PubMed).
-
- IL17F (Interleukin 17F (IL17F))
-
别名
- IL-17F
-
基因ID
- 257630
-
UniProt
- Q7TNI7
-
途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity
抗原 See all IL17F ELISA试剂盒
-
