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ACVA ELISA 试剂盒

The 小鼠 ACVA ELISA Kit (ABIN4885409) is a Colorimetric ELISA Kit designed to quantify 小鼠 ACVA.

产品编号 ABIN4885409

发货至: 中国

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中国
北京 101111

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Beijing Economic Technological Development Area

Room 801-803

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Quick Overview for ACVA ELISA 试剂盒 (ABIN4885409)

抗原

See all ACVA ELISA试剂盒

ACVA (Activin A (ACVA))

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.5 pg/mL - 100 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma, Serum

Discover our top product ACVA ELISA Kit

最低检测浓度

0.5 pg/mL

原理

Mouse Activin A ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method

Quantitative

特异性

This ELISA antibody pair detects Mouse, Human and Rat Activin A beta A subunit. Other species not determined. This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 6Ckine (CCL21), ADAMTS-1 (METH1), Adiponectin (ACRP30), Angiopoietin-3 (ANGPTL1), ANGPTL3, Artemin, CCL28 (MEC), CD36 (SR-B3), Chordin, CRP (C-Reactive Protein), E-Cadherin, Epigen, Epiregulin, Fas (TNFRSF6/Apo-1), Galectin-7, gp130, Granzyme B, Gremlin-1, IFN-gamma R1, IL-17 RB, IL-17B, IL-22, MIP-1 beta (CCL4), MMP-10, MMP-2, MMP-3, PDGF-AA, Persephin, sFRP-3, SLAM (CD150), Sonic Hedgehog NTerminal (Shh-N), TCK-1 (CXCL7), TECK (CCL25), TGF beta 1, Trance (TNFSF11), TREML1 (TLT-1), TWEAK (TNFSF12), VEGF-B, VEGFR2.

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
,
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Activin A (ACVA) ELISA Kit
ACVA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Activin A (ACVA) ELISA Kit
ACVA 适用: 小鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Activin A (ACVA) ELISA Kit
ACVA 适用: 大鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum

Activin A (ACVA) ELISA Kit
ACVA 适用: 绵羊 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate

Activin A (ACVA) ELISA Kit
ACVA 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate

Activin A (ACVA) ELISA Kit
ACVA 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate

Activin A (ACVA) ELISA Kit
ACVA 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate

Activin A (ACVA) ELISA Kit
ACVA 适用: 人 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Activin A (ACVA) ELISA Kit
ACVA 适用: 人 Colorimetric Sandwich ELISA 15 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum

Activin A (ACVA) ELISA Kit
ACVA 适用: 人 Colorimetric Sandwich ELISA

应用备注

Suggested Plasma Dilution: 15 fold

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
抗原 See all ACVA ELISA试剂盒

ACVA (Activin A (ACVA))

别名

Activin A

背景

Gene Symbols: Inhibin beta A chain (Activin beta-A chain)

基因ID

16323

UniProt

Q04998

途径

Hormone Transport, Peptide Hormone Metabolism