ICAM1 ELISA 试剂盒
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- 抗原 See all ICAM1 ELISA试剂盒
- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human ICAM-1 (CD54) SpeedELISA Kit.
- 品牌
- SpeedELISA
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 产品特性
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- Compressed workflow with only one wash step and three incubation steps
- 3 hours total processing time
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Streptavidin-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Capture Antibody
- HRP Conjugated Detection Antibody
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Intercellular Adhesion Molecule 1 (ICAM1) ELISA Kit
ICAM1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 犬 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 兔 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 人 AA 28-480 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 小鼠 AA 28-485 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 大鼠 AA 28-493 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 猴 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitSmall Sample ICAM1 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 70 μL of the Antibody Cocktail to each well.
- Incubate 10 minutes at RT.
- Add 50 μL of sample or standard to each well.
- Incubate 2 h at RT.
- Wash Wells
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
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May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. - 有效期
- 6 months
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- 抗原 See all ICAM1 ELISA试剂盒
- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
- 别名
- ICAM-1 (ICAM1 产品)
- 别名
- BB2 ELISA Kit, CD54 ELISA Kit, P3.58 ELISA Kit, Icam-1 ELISA Kit, Ly-47 ELISA Kit, MALA-2 ELISA Kit, ICAM ELISA Kit, ICAM-1 ELISA Kit, ICAM1 ELISA Kit, intercellular adhesion molecule 1 ELISA Kit, intercellular adhesion molecule-1 ELISA Kit, ICAM1 ELISA Kit, Icam1 ELISA Kit, ICAM-1 ELISA Kit
- 物质类
- Viral Protein
- 基因ID
- 3383
- UniProt
- P05362
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
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