ZAP70 ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 ZAP70.
产品编号 ABIN4885072
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for ZAP70 ELISA 试剂盒 (ABIN4885072)
抗原
See all ZAP70 ELISA试剂盒
ZAP70
(zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
3.75 ng/mL - 250 ng/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Plasma, Serum
-
-
最低检测浓度
- 3.75 ng/mL
-
原理
- Human ZAP70 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
-
Analytical Method
- Quantitative
-
特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin C, AHCY, APE, Bax, BNIP3, Caspase-3, Cathepsin F, CD177, CD96, CLEC4D, C-Peptide, DCBLD2, Dystroglycan, FAM3A, GCNT1, GLA, HAPLN4, hCG alpha, HMGB3, IkB-E, IRF4, KIR3DL1, Klotho beta, Lck, LILRA5, METTL11A, p55PIK, PCDH17, PLA2G4A, RalB, S100A2, S100B, SH2B1, Siglec-3, SOD3, ST8SIA2, Testican 1, TRP14, VAP-B.
-
产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) ELISA Kit
ZAP70 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Suggested Plasma Dilution: 2 fold
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
-
有效期
- 6 months
-
-
- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
-
别名
- ZAP70
-
背景
-
Gene Symbols: SRK, ZAP70
Protein Name (Synonyms): Tyrosine-protein kinase ZAP-70 (EC 2.7.10.2) (70 kDa zeta-chain associated protein) (Syk-related tyrosine kinase)
-
基因ID
- 7535
-
UniProt
- P43403
-
途径
- TCR Signaling, Ubiquitin Proteasome Pathway
抗原 See all ZAP70 ELISA试剂盒
-
You are here:
