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PRSS2 ELISA 试剂盒

PRSS2 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN4884926
发货至: 中国
  • 抗原 See all PRSS2 ELISA试剂盒
    PRSS2 (Protease, serine, 2 (Trypsin 2) (PRSS2))
    适用
    • 2
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human Trypsin 2/PRSS2 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human Trypsin 2.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all PRSS2 ELISA试剂盒
    PRSS2 (Protease, serine, 2 (Trypsin 2) (PRSS2))
    别名
    Trypsin 2 (PRSS2 产品)
    别名
    Dvir\\GJ21501 ELISA Kit, Dvtry-2 ELISA Kit, GJ21501 ELISA Kit, Try2 ELISA Kit, dvir_GLEANR_6854 ELISA Kit, TRY8 ELISA Kit, TRYP ELISA Kit, Ta ELISA Kit, Tesp4 ELISA Kit, Ptryss2 ELISA Kit, TRY2 ELISA Kit, TRYP2 ELISA Kit, trypsin-2 ELISA Kit, Trypsin-2 ELISA Kit, protease, serine 2 ELISA Kit, protease, serine, 2 ELISA Kit, trypsin II ELISA Kit, CpipJ_CPIJ000771 ELISA Kit, CpipJ_CPIJ020109 ELISA Kit, Dvir\Try2 ELISA Kit, Prss2 ELISA Kit, PRSS2 ELISA Kit, trp-ii ELISA Kit
    基因ID
    5645
    UniProt
    P07478
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