TREM2 ELISA 试剂盒
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Quick Overview for TREM2 ELISA 试剂盒 (ABIN4884887)
抗原
See all TREM2 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 28 pg/mL
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原理
- Human TREM-2 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
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Analytical Method
- Quantitative
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特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ACE, Activin RIB, ADAM23, Artemin, Cardiotrophin-1, Cathepsin V, FABP1, FGF-20, GDF-8, HAI-1, IL-27 R alpha, Insulin R, Kallikrein 7, LIF R alpha, Lipocalin-1, LTbR, Mesothelin, MFRP, Neuropilin-2, Neurturin, Nidogen-2, Olfactomedin-2, p53, PDECGF, PDGF-CC, Progranulin, Ret, ROBO4, Semaphorin 6B, Serpin F1, SREC-I, SREC-II, TLR1, TLR3, TPP1, TrkC, TROY, Uromodulin, XIAP.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit
TREM2 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA KitTREM2 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA KitTREM2 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA KitWide Range TREM2 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA KitTREM2 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA KitTREM2 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA KitTREM2 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA KitTREM2 适用: 人 AA 19-167 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA KitTREM2 适用: 人 Colorimetric Sandwich ELISA 156.25 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma, Serum
Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA KitSmall Sample TREM2 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: 2 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
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May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. -
有效期
- 6 months
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- TREM2 (Triggering Receptor Expressed On Myeloid Cells 2 (TREM2))
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别名
- TREM-2
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背景
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Gene Symbols: TREM2
Protein Name (Synonyms): Triggering receptor expressed on myeloid cells 2 (TREM-2) (Triggering receptor expressed on monocytes 2)
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基因ID
- 54209
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UniProt
- Q9NZC2
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