Transferrin Receptor ELISA 试剂盒
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- 抗原 See all Transferrin Receptor (TFRC) ELISA试剂盒
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.819-200 ng/mL
- 最低检测浓度
- 0.819 ng/mL
- 应用范围
- ELISA
- 原理
- Human TfR/Transferrin R/CD71 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human TfR.
- 灵敏度
- 0.81 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Transferrin Receptor (p90, CD71) (TFRC) ELISA Kit
TFRC 适用: 小鼠 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Transferrin Receptor (p90, CD71) (TFRC) ELISA KitTFRC 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Transferrin Receptor (p90, CD71) (TFRC) ELISA KitTFRC 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Transferrin Receptor (p90, CD71) (TFRC) ELISA KitTFRC 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Transferrin Receptor (p90, CD71) (TFRC) ELISA KitTFRC 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Transferrin Receptor (p90, CD71) (TFRC) ELISA KitTFRC 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Transferrin Receptor (p90, CD71) (TFRC) ELISA KitTFRC 适用: 小鼠 Colorimetric Competition ELISA 2.5-50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples200 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all Transferrin Receptor (TFRC) ELISA试剂盒
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
- 别名
- TfR (Transferrin R/CD71) (TFRC 产品)
- 别名
- Trfr ELISA Kit, LOC100219280 ELISA Kit, tfr ELISA Kit, cd71 ELISA Kit, tfr1 ELISA Kit, trfr ELISA Kit, CD71 ELISA Kit, T9 ELISA Kit, TFR ELISA Kit, TFR1 ELISA Kit, TR ELISA Kit, TRFR ELISA Kit, p90 ELISA Kit, 2610028K12Rik ELISA Kit, AI195355 ELISA Kit, AI426448 ELISA Kit, AU015758 ELISA Kit, E430033M20Rik ELISA Kit, Mtvr-1 ELISA Kit, Mtvr1 ELISA Kit, transferrin receptor ELISA Kit, TFRC ELISA Kit, Tfrc ELISA Kit, tfrc ELISA Kit
- 基因ID
- 7037
- UniProt
- P02786
- 途径
- Transition Metal Ion Homeostasis
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