ST6GAL1 ELISA 试剂盒
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Quick Overview for ST6GAL1 ELISA 试剂盒 (ABIN4884659)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.6 ng/mL
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原理
- Human ST6GAL1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin RIIA, Biglycan, Carbonic Anhydrase XIII, Carbonic Anhydrase II, CA72-4, CLEC-2, C-myc, Cystatin D, Erythropoietin, FCRL5/FcRH5, FGF-16, GATA-4, GFR alpha-1, GFR alpha-2, Granzyme B, Granzyme H, HIF-1a, htPAPPA, IFNb, IL-17 RC, IL-19, IL-20 R beta, IL-22, ILT4/CD85d, LAIR2, LSECtin/CLEC4G, Netrin-4, Norrin, NRG1a, PD-L2, PDX-1, Podocalyxin, RGM-C/Hemojuvelin, S100A1, Semaphorin 6A, SLITRK5, SR-AI, Thyroid Peroxidase, Troponin C.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1) ELISA Kit
ST6GAL1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1) ELISA KitST6GAL1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1) ELISA KitSmall Sample ST6GAL1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1) ELISA KitST6GAL1 适用: 人 Colorimetric Sandwich ELISA 3.9-250 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1) ELISA KitST6GAL1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1) ELISA KitSmall Sample ST6GAL1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1) ELISA KitST6GAL1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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应用备注
- Suggested Plasma Dilution: 2 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- ST6GAL1 (ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1))
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别名
- ST6GAL1
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背景
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Gene Symbols: ST6GAL1, SIAT1
Protein Name (Synonyms): Beta-galactoside alpha-2,6-sialyltransferase 1 (Alpha 2,6-ST 1) (EC 2.4.99.1) (B-cell antigen CD75) (CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1) (ST6Gal I) (ST6GalI) (Sialyltransferase 1)
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基因ID
- 6480
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UniProt
- P15907
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