ROBO3 ELISA 试剂盒
The 人 ROBO3 ELISA Kit (ABIN4884368) is a Colorimetric ELISA Kit designed to quantify 人 ROBO3.
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产品编号 ABIN4884368
发货至:
中国
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Our Local Distributor
中国
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北京 101111
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Quick Overview for ROBO3 ELISA 试剂盒 (ABIN4884368)
抗原
See all ROBO3 ELISA试剂盒
ROBO3
(Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.06 ng/mL - 15 ng/mL
应用范围
ELISA
样品类型
Plasma, Cell Culture Supernatant, Serum
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最低检测浓度
- 0.06 ng/mL
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原理
- Human ROBO3 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS-13, Aggrecan, Angiotensinogen, B7-H1, BMPR-IA, BMPR-II, Cadherin-11, CD27, CD6, Ck beta 8-1, CNTF, DNAM-1, CD147, FLRG, Follistatin-like 1, Fractalkine, Galectin-1, GITR Ligand, Granulysin, IL-1 R3, IL-15 R alpha, IL-17E, IL-32 alpha, CHL-1, LRIG3, LRP-6, MEPE, Nectin-4, Periostin, Persephin, Renin, RGM-B, S100 A8, Siglec-7, Syndecan-3, Thrombospondin 2, Thrombospondin 5, Tie-1, ULBP-2.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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应用备注
- Suggested Plasma Dilution: 2 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
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May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. -
有效期
- 6 months
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- ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))
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别名
- ROBO3
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背景
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Gene Symbols: ROBO3
Protein Name (Synonyms): Roundabout homolog 3 (Roundabout-like protein 3)
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基因ID
- 64221
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UniProt
- Q96MS0
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