PPY ELISA 试剂盒
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北京 101111
Quick Overview for PPY ELISA 试剂盒 (ABIN4884226)
抗原
See all PPY ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 4 pg/mL
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原理
- Human Pancreatic polypeptide/ProPPY/PP ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human Coagulation Factor X, CHMP2B, Contactin-3, Cortactin, CrkL, Cyr61/CCN1, DAPP1, Dynactin Subunit 1/DCTN1, DFF45/ICAD, DRAK1, GRAP2, GRK5, Hydroxyacid Oxidase-1/HAO-1, LRIG1, MMP-12, NCK1, Nectin-2/CD112, Nesfatin-1/Nucleobindin-2, Neurogranin, Nrf2, NUDT5, Nucleoporin NUP85, PAR1, Peroxiredoxin 2, PSMA1, PU.1/Spi-1, RalA, RCOR1/CoREST, Serpin B8, SH2D1A, SHP-1, Siglec-6/CD327, SorCS3, THAP11, ULBP-4/RAET1E, UNC5H3, VAMP-1, VAMP-2, PBEF/Visfatin.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Pancreatic Polypeptide (PPY) ELISA Kit
PPY 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 pg/mL - 50 pg/mL Plasma, Serum
Pancreatic Polypeptide (PPY) ELISA KitPPY 适用: 人 Colorimetric Competition ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Pancreatic Polypeptide (PPY) ELISA KitPPY 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Pancreatic Polypeptide (PPY) ELISA KitPPY 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum
Pancreatic Polypeptide (PPY) ELISA KitPPY 适用: 小鼠 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
Pancreatic Polypeptide (PPY) ELISA KitPPY 适用: 人 Colorimetric Competition ELISA 37.0 pg/mL - 3000 pg/mL Plasma, Serum
Pancreatic Polypeptide (PPY) ELISA KitPPY 适用: 大鼠 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
Pancreatic Polypeptide (PPY) ELISA KitSmall Sample PPY 适用: 大鼠 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
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应用备注
- Suggested Plasma Dilution: 2 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- PPY (Pancreatic Polypeptide (PPY))
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别名
- Pancreatic Polypeptide
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背景
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Gene Symbols: PPY, PNP
Protein Name (Synonyms): Pancreatic prohormone (Pancreatic polypeptide) (PP) (Obinepitide) [Cleaved into: Pancreatic hormone (PH) Pancreatic icosapeptide (PI)
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基因ID
- 5539
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UniProt
- P01298
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途径
- Feeding Behaviour
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