PLA2G4A ELISA 试剂盒
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- 抗原 See all PLA2G4A ELISA试剂盒
- PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human PLA2G4A ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes .
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) ELISA Kit
PLA2G4A 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) ELISA KitPLA2G4A 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) ELISA KitPLA2G4A 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) ELISA KitPLA2G4A 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all PLA2G4A ELISA试剂盒
- PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))
- 别名
- PLA2G4A (PLA2G4A 产品)
- 别名
- PLA2G4 ELISA Kit, cPLA2-alpha ELISA Kit, Pla2c ELISA Kit, Pla2g4 ELISA Kit, cPLA2 ELISA Kit, pla2g4 ELISA Kit, CPLA2 ELISA Kit, PLA2G4A ELISA Kit, PLA2 ELISA Kit, PLA2G1B ELISA Kit, cPLA2alpha ELISA Kit, pla2g4a ELISA Kit, si:dkey-97o5.1 ELISA Kit, phospholipase A2 group IVA ELISA Kit, phospholipase A2 group IVA L homeolog ELISA Kit, phospholipase A2, group IVA (cytosolic, calcium-dependent) ELISA Kit, phospholipase A2, group IVAb (cytosolic, calcium-dependent) ELISA Kit, PLA2G4A ELISA Kit, Pla2g4a ELISA Kit, pla2g4a.L ELISA Kit, pla2g4ab ELISA Kit
- 基因ID
- 5321
- UniProt
- P47712
- 途径
- Inositol Metabolic Process, G-protein mediated Events, VEGF Signaling
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