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PCK1 ELISA 试剂盒

PCK1 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN4884106
发货至: 中国
  • 抗原 See all PCK1 ELISA试剂盒
    PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Human PCK1/PEPCK-C ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes .
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all PCK1 ELISA试剂盒
    PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
    别名
    PCK1 (PCK1 产品)
    别名
    PEPCK-C ELISA Kit, PEPCK1 ELISA Kit, PEPCKC ELISA Kit, PEPCK ELISA Kit, PEPCK-M ELISA Kit, PEPCK2 ELISA Kit, 1810010O14Rik ELISA Kit, 9130022B02Rik ELISA Kit, cb856 ELISA Kit, cb924 ELISA Kit, fj93h11 ELISA Kit, zgc:63869 ELISA Kit, wu:fc51c05 ELISA Kit, wu:fj93h11 ELISA Kit, pepck-c ELISA Kit, pepck1 ELISA Kit, pepckc ELISA Kit, PCK1 ELISA Kit, PPCK1 ELISA Kit, AI265463 ELISA Kit, Pck-1 ELISA Kit, GTP ELISA Kit, PCK ELISA Kit, Pepck ELISA Kit, RATPEPCK ELISA Kit, Ppc1C ELISA Kit, PHOSPHOENOLPYRUVATE CARBOXYKINASE ELISA Kit, T28I19.150 ELISA Kit, T28I19_150 ELISA Kit, phosphoenolpyruvate carboxykinase 1 ELISA Kit, 143299_at ELISA Kit, CG10924 ELISA Kit, CG17725 ELISA Kit, Dmel\\CG17725 ELISA Kit, Dromel_CG17725_FBtr0086701_pepck_mORF ELISA Kit, dPEPCK ELISA Kit, pepck ELISA Kit, PEPC ELISA Kit, PEPCase ELISA Kit, ppc ELISA Kit, phosphoenolpyruvate carboxykinase 1 ELISA Kit, phosphoenolpyruvate carboxykinase 2, mitochondrial ELISA Kit, phosphoenolpyruvate carboxykinase 2 (mitochondrial) ELISA Kit, phosphoenolpyruvate carboxykinase 1 (soluble) ELISA Kit, phosphoenolpyruvate carboxykinase 1 S homeolog ELISA Kit, phosphoenolpyruvate carboxykinase, cytosolic [GTP] ELISA Kit, phosphoenolpyruvate carboxykinase 1, cytosolic ELISA Kit, phosphoenolpyruvate carboxylase 7 ELISA Kit, Phosphoenolpyruvate carboxykinase ELISA Kit, phosphoenolpyruvate carboxylase ELISA Kit, PCK1 ELISA Kit, PCK2 ELISA Kit, Pck2 ELISA Kit, pck1 ELISA Kit, pck1.S ELISA Kit, LOC100634531 ELISA Kit, Pck1 ELISA Kit, pep7 ELISA Kit, Pepck ELISA Kit, LOC107777405 ELISA Kit
    基因ID
    5105
    UniProt
    P35558
    途径
    Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
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