NRF2 ELISA 试剂盒
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Quick Overview for NRF2 ELISA 试剂盒 (ABIN4883982)
抗原
See all NRF2 (NFE2L2) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 14 pg/mL
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原理
- Human NRF2 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects human Nrf-2. Other species not determined.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) ELISA Kit
NFE2L2 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) ELISA KitNFE2L2 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) ELISA KitNFE2L2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) ELISA KitNFE2L2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) ELISA KitNFE2L2 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) ELISA KitNFE2L2 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) ELISA KitNFE2L2 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) ELISA KitNFE2L2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) ELISA KitNFE2L2 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) ELISA KitNFE2L2 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
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应用备注
- Suggested Plasma Dilution: 2 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
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别名
- Nrf2
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背景
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Gene Symbols: NFE2L2, NRF2
Protein Name (Synonyms): Nuclear factor erythroid 2-related factor 2 (NF-E2-related factor 2) (NFE2-related factor 2) (HEBP1) (Nuclear factor, erythroid derived 2, like 2)
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基因ID
- 4780
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UniProt
- Q16236
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途径
- ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling
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