HNF4A ELISA 试剂盒
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- 抗原 See all HNF4A ELISA试剂盒
- HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human HNF-4 alpha/NR2A1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes .
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling HNF4A ELISA Kit
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- Discover our top product HNF4A ELISA Kit
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Hepatocyte Nuclear Factor 4, alpha (HNF4A) ELISA Kit
HNF4A 适用: 小鼠 Colorimetric Sandwich ELISA 39.063 pg/mL - 2500 pg/mL Plasma, Serum, Tissue Homogenate
Hepatocyte Nuclear Factor 4, alpha (HNF4A) ELISA KitHNF4A 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all HNF4A ELISA试剂盒
- HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))
- 别名
- HNF-4 alpha (HNF4A 产品)
- 别名
- HNF4A ELISA Kit, HNF4alpha ELISA Kit, CG9310 ELISA Kit, DmHNF4 ELISA Kit, Dmel\\CG9310 ELISA Kit, HNF-4 ELISA Kit, HNF-4(D) ELISA Kit, HNF4 ELISA Kit, Hnf-4 ELISA Kit, Hnf-4h ELISA Kit, NR2A4 ELISA Kit, dHNF-4 ELISA Kit, dHNF4 ELISA Kit, Hnf4 ELISA Kit, Hnf4alpha ELISA Kit, MODY1 ELISA Kit, Nr2a1 ELISA Kit, Tcf14 ELISA Kit, HNF4a7 ELISA Kit, HNF4a8 ELISA Kit, HNF4a9 ELISA Kit, MODY ELISA Kit, NR2A1 ELISA Kit, NR2A21 ELISA Kit, TCF ELISA Kit, TCF14 ELISA Kit, HNF-4alpha ELISA Kit, fb58h09 ELISA Kit, id:ibd1279 ELISA Kit, wu:fb58h09 ELISA Kit, hnf4 ELISA Kit, nr2a1 ELISA Kit, hepatocyte nuclear factor 4 alpha ELISA Kit, Hepatocyte nuclear factor 4 ELISA Kit, hepatic nuclear factor 4, alpha ELISA Kit, hepatocyte nuclear factor 4, alpha ELISA Kit, hepatocyte nuclear factor 4 alpha S homeolog ELISA Kit, HNF4A ELISA Kit, hnf4a ELISA Kit, Hnf4 ELISA Kit, Hnf4a ELISA Kit, hnf4a.S ELISA Kit
- 基因ID
- 3172
- UniProt
- P41235
- 途径
- AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process
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