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GATA4 ELISA 试剂盒

GATA4 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN4883026
发货至: 中国
  • 抗原 See all GATA4 ELISA试剂盒
    GATA4 (GATA Binding Protein 4 (GATA4))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Human GATA4 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes human GATA4. Other species not yet determined.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all GATA4 ELISA试剂盒
    GATA4 (GATA Binding Protein 4 (GATA4))
    别名
    GATA4 (GATA4 产品)
    别名
    gata-4 ELISA Kit, xgata4 ELISA Kit, xGATA-4 ELISA Kit, MGC81427 ELISA Kit, GATA4 ELISA Kit, GATA-4 ELISA Kit, Gata4 ELISA Kit, gata4 ELISA Kit, ASD2 ELISA Kit, VSD1 ELISA Kit, Gata-4 ELISA Kit, gata ELISA Kit, GATA binding protein 4 ELISA Kit, GATA binding protein 4 L homeolog ELISA Kit, glutamyl-tRNA(Gln) amidotransferase subunit A ELISA Kit, gata4 transcription factor ELISA Kit, GATA binding protein 4 S homeolog ELISA Kit, GATA4 ELISA Kit, gata4.L ELISA Kit, gata4 ELISA Kit, gatA4 ELISA Kit, Gata4 ELISA Kit, gata4.S ELISA Kit
    基因ID
    2626
    UniProt
    P43694
    途径
    Peptide Hormone Metabolism, Carbohydrate Homeostasis
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