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Galectin 2 ELISA 试剂盒

This Colorimetric ELISA kit is designed for the quantitative measurement of 人 Galectin 2.
产品编号 ABIN4882972
发货至: 中国
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Quick Overview for Galectin 2 ELISA 试剂盒 (ABIN4882972)

抗原

See all Galectin 2 (LGALS2) ELISA试剂盒
Galectin 2 (LGALS2) (Lectin, Galactoside-Binding, Soluble, 2 (LGALS2))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

22.3 pg/mL - 6000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma, Serum
  • 最低检测浓度

    22.3 pg/mL

    原理

    Human Galectin-2 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

    Analytical Method

    Quantitative

    特异性

    This ELISA antibody pair detects human Galectin-2. Other species not determined.

    产品特性

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    组件

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate< div class='ty-product-feature'>< div class='ty-product-feature__label'>Other Materials Required< div class='ty-product-feature__value'>
      • Distilled or deionized water
      • Precision pipettes to deliver 2 μl to 1 μl volumes
      • Adjustable 1-25 μl pipettes for reagent preparation
      • 100 μl and 1 liter graduated cylinders
      • Tubes to prepare standard and sample dilutions
      • Absorbent paper
      • Microplate reader capable of measuring absorbance at 450nm
      • Log-log graph paper or computer and software for ELISA data analysis
      ,
      • Pre-Coated 96-well Strip Microplate
      • Wash Buffer
      • Stop Solution
      • Assay Diluent(s)
      • Lyophilized Standard
      • Biotinylated Detection Antibody
      • Streptavidin-Conjugated HRP
      • TMB One-Step Substrate< div class='ty-product-feature'>< div class='ty-product-feature__label'>Other Materials Required< div class='ty-product-feature__value'>
        • Distilled or deionized water
        • Precision pipettes to deliver 2 μl to 1 μl volumes
        • Adjustable 1-25 μl pipettes for reagent preparation
        • 100 μl and 1 liter graduated cylinders
        • Tubes to prepare standard and sample dilutions
        • Absorbent paper
        • Microplate reader capable of measuring absorbance at 450nm
        • Log-log graph paper or computer and software for ELISA data analysis

    试剂未包括

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 应用备注

    Suggested Plasma Dilution: 2 - 20 fold

    板类型

    Pre-coated

    实验流程

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    限制

    仅限研究用
  • 储存条件

    -20 °C

    储存方法

    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C.

    有效期

    6 months
  • 抗原 See all Galectin 2 (LGALS2) ELISA试剂盒

    Galectin 2 (LGALS2) (Lectin, Galactoside-Binding, Soluble, 2 (LGALS2))

    别名

    Galectin-2

    背景

    Gene Symbols: LGALS2

    Protein Name (Synonyms): Galectin-2 (Gal-2) (Beta-galactoside-binding lectin L-14-II) (HL14) (Lactose-binding lectin 2) (S-Lac lectin 2)

    基因ID

    3957

    UniProt

    P05162
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